@article{e3323e7ea3994105b1256f69298e61d2,
title = "The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty",
abstract = "Guo et al. provide a transcriptional cell atlas of the developing human testis during puberty, revealing dramatic developmental changes in both germ and somatic niche cell lineages. Furthermore, germ cells and Sertoli cells from testosterone-suppressed transfemale testes display partial developmental reversal, revealing critical roles for testosterone in maintaining testis maturation.",
keywords = "Leydig cell, peritubular myoid cell, puberty, Sertoli cell, single cell sequencing, spermatogonial stem cell, state 0, testis maturation, testosterone, transfemale",
author = "DonorConnect and Jingtao Guo and Xichen Nie and Maria Giebler and Hana Mlcochova and Yueqi Wang and Grow, {Edward J.} and Robin Kim and Melissa Tharmalingam and Gabriele Matilionyte and Cecilia Lindskog and Carrell, {Douglas T.} and Mitchell, {Rod T.} and Anne Goriely and Hotaling, {James M.} and Cairns, {Bradley R.}",
note = "Funding Information: We are especially grateful to the donors and their families, who made this work possible. We thank Brian Dalley and Opal Allen (Genomics Core at Huntsman Cancer Institute) for sequencing expertise, Chris Conley and Tim Parnell (Bioinformatics Core at Huntsman Cancer Institute) for bioinformatics assistance, the Huntsman Cancer Institute Biorepository and Molecular Pathology Shared Resource for tissue sectioning, and DonorConnect staff for family consents and sample handling. Imaging was performed at the Fluorescence Microscopy Core Facility at the Health Sciences Cores at University of Utah, supported by an NCRR Shared Equipment Grant 1S10RR024761-01. Financial support was from Howard Hughes Medical Institute to B.R.C. and P30CA042014 from the National Cancer Institute to Huntsman Cancer Institute for core facilities. The Wellcome, UK (Senior Investigator Award 102731 to Prof. Andrew Wilkie) supports A.G. and H.M.; A.G. is supported by the National Institute for Health Research (NIHR) Oxford Biomedical Research Centre (BRC), UK; M.G. is supported by the EU(ESF)-funded Research Exchange Network HAL-OX ZS/2016/08/80642 of the Martin Luther University Halle-Wittenberg, Germany; and the Wolfson Imaging Centre (Oxford) is supported by the WIMM Strategic Alliance (G0902418 and MC_UU_12025). C.L. is supported by the Knut and Alice Wallenberg Foundation. R.T.M. is supported by a UKRI Future Leaders Fellowship (MR/S017151/1) and MRC Centre for Reproductive Health Centre Grant (MR/N022556/1). B.R.C. and J.G. conceived the project, designed the genomics and computational analysis, and advised on other sections; J.G. conducted genomics experiments and computational analyses with help from X.N.; Y.W. contributed to website construction with help from J.G.; sample acquisition was led by J.M.H. and R.K. with input from D.T.C. B.R.C. and J.G.; samples were transferred and processed by J.G. E.J.G. and X.N.; C.L. provided HPA antibodies and contributed to antibody validation; J.G. X.N. M.G. H.M. M.T. and G.M. performed IHC/IF experiments that were supervised and coordinated by A.G. and R.T.M.; the manuscript was written by J.G. and B.R.C. with input from R.T.M. and A.G. and agreement of all authors. The authors declare no competing interests. Funding Information: We are especially grateful to the donors and their families, who made this work possible. We thank Brian Dalley and Opal Allen (Genomics Core at Huntsman Cancer Institute) for sequencing expertise, Chris Conley and Tim Parnell (Bioinformatics Core at Huntsman Cancer Institute) for bioinformatics assistance, the Huntsman Cancer Institute Biorepository and Molecular Pathology Shared Resource for tissue sectioning, and DonorConnect staff for family consents and sample handling. Imaging was performed at the Fluorescence Microscopy Core Facility at the Health Sciences Cores at University of Utah, supported by an NCRR Shared Equipment Grant 1S10RR024761-01 . Financial support was from Howard Hughes Medical Institute to B.R.C. and P30CA042014 from the National Cancer Institute to Huntsman Cancer Institute for core facilities. The Wellcome, UK (Senior Investigator Award 102731 to Prof. Andrew Wilkie) supports A.G. and H.M.; A.G. is supported by the National Institute for Health Research (NIHR) Oxford Biomedical Research Centre (BRC), UK; M.G. is supported by the EU(ESF)-funded Research Exchange Network HAL-OX ZS/2016/08/80642 of the Martin Luther University Halle-Wittenberg, Germany ; and the Wolfson Imaging Centre (Oxford) is supported by the WIMM Strategic Alliance ( G0902418 and MC_UU_12025 ). C.L. is supported by the Knut and Alice Wallenberg Foundation . R.T.M. is supported by a UKRI Future Leaders Fellowship ( MR/S017151/1 ) and MRC Centre for Reproductive Health Centre Grant ( MR/N022556/1 ). Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = feb,
day = "6",
doi = "10.1016/j.stem.2019.12.005",
language = "English (US)",
volume = "26",
pages = "262--276.e4",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "2",
}