The dynamin middle domain is critical for tetramerization and higher-order self-assembly

Rajesh Ramachandran, Mark Surka, Joshua S. Chappie, Douglas M. Fowler, Ted R. Foss, Byeong Doo Song, Sandra L. Schmid

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The large multidomain GTPase dynamin self-assembles around the necks of deeply invaginated coated pits at the plasma membrane and catalyzes vesicle scission by mechanisms that are not yet completely understood. Although a structural role for the 'middle' domain in dynamin function has been suggested, it has not been experimentally established. Furthermore, it is not clear whether this putative function pertains to dynamin structure in the unassembled state or to its higher-order self-assembly or both. Here, we demonstrate that two mutations in this domain, R361S and R399A, disrupt the tetrameric structure of dynamin in the unassembled state and impair its ability to stably bind to and nucleate higher-order self-assembly on membranes. Consequently, these mutations also impair dynamin's assembly-dependent stimulated GTPase activity.

Original languageEnglish (US)
Pages (from-to)559-566
Number of pages8
JournalEMBO Journal
Volume26
Issue number2
DOIs
StatePublished - Jan 24 2007

Fingerprint

Dynamins
Self assembly
GTP Phosphohydrolases
Mutation
Cell membranes
Neck
Cell Membrane
Membranes

Keywords

  • Dynamin
  • Endocytosis
  • GTPase
  • Middle domain
  • Structure

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Ramachandran, R., Surka, M., Chappie, J. S., Fowler, D. M., Foss, T. R., Song, B. D., & Schmid, S. L. (2007). The dynamin middle domain is critical for tetramerization and higher-order self-assembly. EMBO Journal, 26(2), 559-566. https://doi.org/10.1038/sj.emboj.7601491

The dynamin middle domain is critical for tetramerization and higher-order self-assembly. / Ramachandran, Rajesh; Surka, Mark; Chappie, Joshua S.; Fowler, Douglas M.; Foss, Ted R.; Song, Byeong Doo; Schmid, Sandra L.

In: EMBO Journal, Vol. 26, No. 2, 24.01.2007, p. 559-566.

Research output: Contribution to journalArticle

Ramachandran, R, Surka, M, Chappie, JS, Fowler, DM, Foss, TR, Song, BD & Schmid, SL 2007, 'The dynamin middle domain is critical for tetramerization and higher-order self-assembly', EMBO Journal, vol. 26, no. 2, pp. 559-566. https://doi.org/10.1038/sj.emboj.7601491
Ramachandran R, Surka M, Chappie JS, Fowler DM, Foss TR, Song BD et al. The dynamin middle domain is critical for tetramerization and higher-order self-assembly. EMBO Journal. 2007 Jan 24;26(2):559-566. https://doi.org/10.1038/sj.emboj.7601491
Ramachandran, Rajesh ; Surka, Mark ; Chappie, Joshua S. ; Fowler, Douglas M. ; Foss, Ted R. ; Song, Byeong Doo ; Schmid, Sandra L. / The dynamin middle domain is critical for tetramerization and higher-order self-assembly. In: EMBO Journal. 2007 ; Vol. 26, No. 2. pp. 559-566.
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