The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella

Thomas Heuser, Milen Raytchev, Jeremy Krell, Mary E. Porter, Daniela Nicastro

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Cilia and flagella are highly conserved microtubule (MT)-based organelles with motile and sensory functions, and ciliary defects have been linked to several human diseases. The 9 + 2 structure of motile axonemes contains nine MT doublets interconnected by nexin links, which surround a central pair of singlet MTs. Motility is generated by the orchestrated activity of thousands of dynein motors, which drive interdoublet sliding. A key regulator of motor activity is the dynein regulatory complex (DRC), but detailed structural information is lacking. Using cryoelectron tomography of wild-type and mutant axonemes from Chlamydomonas reinhardtii, we visualized the DRC in situ at molecular resolution. We present the three-dimensional structure of the DRC, including a model for its subunit organization and intermolecular connections that establish the DRC as a major regulatory node. We further demonstrate that the DRC is the nexin link, which is thought to be critical for the generation of axonemal bending.

Original languageEnglish (US)
Pages (from-to)921-933
Number of pages13
JournalJournal of Cell Biology
Volume187
Issue number6
DOIs
StatePublished - Dec 14 2009

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Dyneins
Flagella
Cilia
Axoneme
Microtubules
Chlamydomonas reinhardtii
Organelles
Motor Activity
Tomography

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella. / Heuser, Thomas; Raytchev, Milen; Krell, Jeremy; Porter, Mary E.; Nicastro, Daniela.

In: Journal of Cell Biology, Vol. 187, No. 6, 14.12.2009, p. 921-933.

Research output: Contribution to journalArticle

Heuser, Thomas ; Raytchev, Milen ; Krell, Jeremy ; Porter, Mary E. ; Nicastro, Daniela. / The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella. In: Journal of Cell Biology. 2009 ; Vol. 187, No. 6. pp. 921-933.
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