The effect of anti-immunoglobulin antibodies on the in vitro proliferation and differentiation of normal and neoplastic murine B cells

P. C. Isakson, K. A. Krolick, J. W. Uhr, E. S. Vitetta

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38 Scopus citations


In these studies, we have compared the effects of anti-μ and anti-δ antibodies on normal and neoplastic murine B cells. Spleen cells from normal BALB/c mice or from mice carrying the BCL1 tumor were treated with F(ab')2 fragments of affinity purified anti-μ or anti-δ antibodies and the effect of the antibodies on cell proliferation was assessed. F(ab')2 fragments of both antibodies induced proliferation of normal adult B cells but inhibited (anti-μ) or had no effect (anti-δ) on proliferation of BCL1 cells. In addition, both antibodies further enhanced the LPS-induced proliferative response of cells from adult mice. In contrast, the LPS responses of neonatal cells and BCL1 cells were inhibited by F(ab')2 anti-μ antibodies. These data indicate that interaction of antibody with surface IgD as well as surface IgM can activate adult B cells. In addition, the results provide further evidence that BCL1 cells are neoplastic analogs of immature B cells since the tumor cells, like neonatal cells, are not activated after treatment with anti-Ig antibodies.

Original languageEnglish (US)
Pages (from-to)886-892
Number of pages7
JournalJournal of Immunology
Issue number2
StatePublished - Jan 1 1980


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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