The effect of anti-immunoglobulin antibodies on the in vitro proliferation and differentiation of normal and neoplastic murine B cells

P. C. Isakson, K. A. Krolick, J. W. Uhr, E. S. Vitetta

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Abstract

In these studies, we have compared the effects of anti-μ and anti-δ antibodies on normal and neoplastic murine B cells. Spleen cells from normal BALB/c mice or from mice carrying the BCL1 tumor were treated with F(ab')2 fragments of affinity purified anti-μ or anti-δ antibodies and the effect of the antibodies on cell proliferation was assessed. F(ab')2 fragments of both antibodies induced proliferation of normal adult B cells but inhibited (anti-μ) or had no effect (anti-δ) on proliferation of BCL1 cells. In addition, both antibodies further enhanced the LPS-induced proliferative response of cells from adult mice. In contrast, the LPS responses of neonatal cells and BCL1 cells were inhibited by F(ab')2 anti-μ antibodies. These data indicate that interaction of antibody with surface IgD as well as surface IgM can activate adult B cells. In addition, the results provide further evidence that BCL1 cells are neoplastic analogs of immature B cells since the tumor cells, like neonatal cells, are not activated after treatment with anti-Ig antibodies.

Original languageEnglish (US)
Pages (from-to)886-892
Number of pages7
JournalJournal of Immunology
Volume125
Issue number2
StatePublished - 1980

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Immunoglobulins
Anti-Idiotypic Antibodies
B-Lymphocytes
Antibodies
Cell Proliferation
Immunoglobulin D
Immunoglobulin Fragments
B-Lymphoid Precursor Cells
In Vitro Techniques
Immunoglobulin M
Neoplasms
Spleen

ASJC Scopus subject areas

  • Immunology

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The effect of anti-immunoglobulin antibodies on the in vitro proliferation and differentiation of normal and neoplastic murine B cells. / Isakson, P. C.; Krolick, K. A.; Uhr, J. W.; Vitetta, E. S.

In: Journal of Immunology, Vol. 125, No. 2, 1980, p. 886-892.

Research output: Contribution to journalArticle

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AU - Vitetta, E. S.

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AB - In these studies, we have compared the effects of anti-μ and anti-δ antibodies on normal and neoplastic murine B cells. Spleen cells from normal BALB/c mice or from mice carrying the BCL1 tumor were treated with F(ab')2 fragments of affinity purified anti-μ or anti-δ antibodies and the effect of the antibodies on cell proliferation was assessed. F(ab')2 fragments of both antibodies induced proliferation of normal adult B cells but inhibited (anti-μ) or had no effect (anti-δ) on proliferation of BCL1 cells. In addition, both antibodies further enhanced the LPS-induced proliferative response of cells from adult mice. In contrast, the LPS responses of neonatal cells and BCL1 cells were inhibited by F(ab')2 anti-μ antibodies. These data indicate that interaction of antibody with surface IgD as well as surface IgM can activate adult B cells. In addition, the results provide further evidence that BCL1 cells are neoplastic analogs of immature B cells since the tumor cells, like neonatal cells, are not activated after treatment with anti-Ig antibodies.

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