The effect of chronic graft-versus-host disease on b cell development

Kathy L. Schreiber, James Forman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Chronic graft-versus-host disease often results in a combined deficiency of humoral and cell-mediated immunity. Clinical and experimental studies have suggested that the decrease in B cell responsiveness is due to a failure of B cell production in the bone marrow, intrinsic B cell defects, excessive suppressor T cell activity, and deficient T helper activity. In the present study, we analyze the basis of B cell immunodeficiency in C.B-20→(C.B-20×B10.D2)Fi animals afflicted with chronic GVHD. The initial decline in B cell production in the BM accounts for the early reduction in the number of B cells in the spleen and BM. Later, as B cells appear in near-normal numbers in the BM, the spleen and lymph node are repopulated by the newly derived B cells. Associated with the appearance of B cells in the BM and spleen is the ability to respond to lipopolysaccharide. In contrast, both B cell populations are severely diminished in their ability to proliferate in response to agar-derived mitogens to form colonies (CFU-B). The reduction in the CFU-B response is most likely a consequence of an inherent B cell defect, since purification of C.B-20→Fi splenic B cells does not restore the colonyforming potential. Unlike BM and splenic B cells, LN B cells are unable to respond to either mitogen. Taken together, these results imply that a population of B cells derived from a distinct lineage and/or B cell maturation is defective in mice undergoing GVHD.

Original languageEnglish (US)
Pages (from-to)597-604
Number of pages8
JournalTransplantation
Volume55
Issue number3
DOIs
StatePublished - Mar 1993

ASJC Scopus subject areas

  • Transplantation

Fingerprint Dive into the research topics of 'The effect of chronic graft-versus-host disease on b cell development'. Together they form a unique fingerprint.

  • Cite this