The effect of cyclophosphamide on development of experimental cholesteatoma

P. H. Pownell, C. G. Wright, K. S. Robinson, W. L. Meyerhoff

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Recent work has shown that middle ear application of propylene glycol in chinchillas produces an inflammatory reaction resulting in cholesteatoma formation in 50% to 70% of animals. This study was done to determine if cyclophosphamide, an immune suppressor and anti-inflammatory agent, is capable of inhibiting cholesteatoma development in the animal model. Methods: Ten adult chinchillas received systemic cyclophosphamide (20 mg/kg per day) for 14 days. On days 5, 8, and 11 of that period, the animals also received bilateral middle ear applications of 60% propylene glycol (0.2 mL per ear). Four weeks after the first propylene glycol application, the animals were killed for histologic evaluation. Results: Eight of the 10 animals survived the full 4-week study period, providing 16 temporal bones for evaluation. It was found that cyclophosphamide reduced the leukocyte counts; however, middle ear inflammation appeared unaffected. Cholesteatoma occurred in eight (50%) of the 16 ears studied, and histologic findings were essentially identical to those previously seen in animals given propylene glycol alone. Conclusion: Under the conditions of this study, cyclophosphamide had no effect on cholesteatoma development in the animal model.

Original languageEnglish (US)
Pages (from-to)1114-1116
Number of pages3
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume120
Issue number10
StatePublished - 1994

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Cholesteatoma
Propylene Glycol
Cyclophosphamide
Chinchilla
Middle Ear
Ear
Animal Models
Temporal Bone
Otitis Media
Leukocyte Count
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Pownell, P. H., Wright, C. G., Robinson, K. S., & Meyerhoff, W. L. (1994). The effect of cyclophosphamide on development of experimental cholesteatoma. Archives of Otolaryngology - Head and Neck Surgery, 120(10), 1114-1116.

The effect of cyclophosphamide on development of experimental cholesteatoma. / Pownell, P. H.; Wright, C. G.; Robinson, K. S.; Meyerhoff, W. L.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 120, No. 10, 1994, p. 1114-1116.

Research output: Contribution to journalArticle

Pownell, PH, Wright, CG, Robinson, KS & Meyerhoff, WL 1994, 'The effect of cyclophosphamide on development of experimental cholesteatoma', Archives of Otolaryngology - Head and Neck Surgery, vol. 120, no. 10, pp. 1114-1116.
Pownell, P. H. ; Wright, C. G. ; Robinson, K. S. ; Meyerhoff, W. L. / The effect of cyclophosphamide on development of experimental cholesteatoma. In: Archives of Otolaryngology - Head and Neck Surgery. 1994 ; Vol. 120, No. 10. pp. 1114-1116.
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