The effect of cytochalasin D on steroid production and stress fiber organization in cultured bovine adrenocortical cells

W. E. Rainey, J. W. Shay, J. I. Mason

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The effects of cytochalasin D on basal and adrenocorticotropin (ACTH)-stimulated steroid production as well as stress fiber organization by bovine adrenocortical (BAC) cells grown in monolayer culture have been investigated. Corticosteroid and pregnenolone release was determined by use of radioimmunoassay. The addition of ACTH (1 nM) produced a 30-fold increase in steroid release (principally corticosterone) in a 2 h period. Cytochalasin D (1-10 μM) had no effect on ACTH-stimulated release of corticosteroids. Basal steroid release was elevated by cytochalasin D with lower concentrations being more effective. In addition, hormonal stimulation of pregnenolone formation, the initial step in cholesterol metabolism leading to corticosteroid production, was also unaffected by cytochalasin D (1-50 μM) addition. Observation of stress fibers by fluorescence microscopy using the probe NDB-phalladin revealed that cytochalasin D (10 and 50 μM) caused an aggregation of actin-containing filaments into stellate foci within the cytoplasm. This was confirmed by electron microscopic examination of the cells. ACTH, however, had no observable effect on stress fiber organization or cell morphology. These results differ from the inhibitory effect of cytochalasin on steroid production observed using Y-1 adrenal tumor cells and rat adrenal cells in primary culture. BAC cells in contrast to Y-1 adrenal tumor cells differ in non-esterified cholesterol content and their morphological response to ACTH treatment. We suggest that these differences may influence the lack of inhibition of cytochalasin D on ACTH-stimulated steroid release.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume35
Issue number2-3
DOIs
StatePublished - 1984

Fingerprint

Cytochalasin D
Stress Fibers
Adrenocorticotropic Hormone
Steroids
Fibers
Pregnenolone
Adrenal Cortex Hormones
Glandular and Epithelial Neoplasms
Cells
Cell culture
Tumors
Cholesterol
Cytochalasins
Primary Cell Culture
Fluorescence microscopy
Corticosterone
Actin Cytoskeleton
Fluorescence Microscopy
Metabolism
Radioimmunoassay

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The effect of cytochalasin D on steroid production and stress fiber organization in cultured bovine adrenocortical cells. / Rainey, W. E.; Shay, J. W.; Mason, J. I.

In: Molecular and Cellular Endocrinology, Vol. 35, No. 2-3, 1984, p. 189-197.

Research output: Contribution to journalArticle

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