The effect of flow on blood oxygen level dependent (R2*) MRI of orthotopic lung tumors

Heling Zhou, Olivier Belzile, Zhang Zhang, Jo Wagner, Chul Ahn, James A Richardson, Debabrata Saha, Rolf A Brekken, Ralph P Mason

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: Blood oxygen level dependent (BOLD) MRI based on R2* measurements can provide insights into tumor vascular oxygenation. However, measurements are susceptible to blood flow, which may vary accompanying a hyperoxic gas challenge. We investigated flow sensitivity by comparing R2* measurements with and without flow suppression (fs) in 2 orthotopic lung xenograft tumor models. Methods: H460 (n = 20) and A549 (n = 20) human lung tumor xenografts were induced by surgical implantation of cancer cells in the right lung of nude rats. MRI was performed at 4.7T after tumors reached 5 to 8 mm in diameter. A multiecho gradient echo MRI sequence was acquired with and without spatial saturation bands on each side of the imaging plane to evaluate the effect of flow on R2*. fs and non-fs R2* MRI measurements were interleaved during an oxygen breathing challenge (from air to 100% O2). T2*-weighted signal intensity changes (ΔSI(%)) and R2* measurements were obtained for regions of interest and on a voxel-by-voxel basis and discrepancies quantified with Bland–Altman analysis. Results: Flow suppression affected ΔSI(%) and R2* measurements in each tumor model. Average discrepancy and limits of agreement from Bland–Altman analyses revealed greater flow-related bias in A549 than H460. Conclusion: The effect of flow on R2*, and hence BOLD, was tumor model dependent with measurements being more sensitive in well-perfused A549 tumors.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StatePublished - Jan 1 2019

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Oxygen
Lung
Neoplasms
Heterografts
Nude Rats
Blood Vessels
Respiration
Gases
Air

Keywords

  • blood oxygen level dependent (BOLD)
  • flow-suppression
  • hypoxia
  • lung cancer
  • oxygen-sensitive MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

@article{e8af530b46fc4891901f1bb049586e28,
title = "The effect of flow on blood oxygen level dependent (R2*) MRI of orthotopic lung tumors",
abstract = "Purpose: Blood oxygen level dependent (BOLD) MRI based on R2* measurements can provide insights into tumor vascular oxygenation. However, measurements are susceptible to blood flow, which may vary accompanying a hyperoxic gas challenge. We investigated flow sensitivity by comparing R2* measurements with and without flow suppression (fs) in 2 orthotopic lung xenograft tumor models. Methods: H460 (n = 20) and A549 (n = 20) human lung tumor xenografts were induced by surgical implantation of cancer cells in the right lung of nude rats. MRI was performed at 4.7T after tumors reached 5 to 8 mm in diameter. A multiecho gradient echo MRI sequence was acquired with and without spatial saturation bands on each side of the imaging plane to evaluate the effect of flow on R2*. fs and non-fs R2* MRI measurements were interleaved during an oxygen breathing challenge (from air to 100{\%} O2). T2*-weighted signal intensity changes (ΔSI({\%})) and R2* measurements were obtained for regions of interest and on a voxel-by-voxel basis and discrepancies quantified with Bland–Altman analysis. Results: Flow suppression affected ΔSI({\%}) and R2* measurements in each tumor model. Average discrepancy and limits of agreement from Bland–Altman analyses revealed greater flow-related bias in A549 than H460. Conclusion: The effect of flow on R2*, and hence BOLD, was tumor model dependent with measurements being more sensitive in well-perfused A549 tumors.",
keywords = "blood oxygen level dependent (BOLD), flow-suppression, hypoxia, lung cancer, oxygen-sensitive MRI",
author = "Heling Zhou and Olivier Belzile and Zhang Zhang and Jo Wagner and Chul Ahn and Richardson, {James A} and Debabrata Saha and Brekken, {Rolf A} and Mason, {Ralph P}",
year = "2019",
month = "1",
day = "1",
doi = "10.1002/mrm.27661",
language = "English (US)",
journal = "Magnetic Resonance in Medicine",
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T1 - The effect of flow on blood oxygen level dependent (R2*) MRI of orthotopic lung tumors

AU - Zhou, Heling

AU - Belzile, Olivier

AU - Zhang, Zhang

AU - Wagner, Jo

AU - Ahn, Chul

AU - Richardson, James A

AU - Saha, Debabrata

AU - Brekken, Rolf A

AU - Mason, Ralph P

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Purpose: Blood oxygen level dependent (BOLD) MRI based on R2* measurements can provide insights into tumor vascular oxygenation. However, measurements are susceptible to blood flow, which may vary accompanying a hyperoxic gas challenge. We investigated flow sensitivity by comparing R2* measurements with and without flow suppression (fs) in 2 orthotopic lung xenograft tumor models. Methods: H460 (n = 20) and A549 (n = 20) human lung tumor xenografts were induced by surgical implantation of cancer cells in the right lung of nude rats. MRI was performed at 4.7T after tumors reached 5 to 8 mm in diameter. A multiecho gradient echo MRI sequence was acquired with and without spatial saturation bands on each side of the imaging plane to evaluate the effect of flow on R2*. fs and non-fs R2* MRI measurements were interleaved during an oxygen breathing challenge (from air to 100% O2). T2*-weighted signal intensity changes (ΔSI(%)) and R2* measurements were obtained for regions of interest and on a voxel-by-voxel basis and discrepancies quantified with Bland–Altman analysis. Results: Flow suppression affected ΔSI(%) and R2* measurements in each tumor model. Average discrepancy and limits of agreement from Bland–Altman analyses revealed greater flow-related bias in A549 than H460. Conclusion: The effect of flow on R2*, and hence BOLD, was tumor model dependent with measurements being more sensitive in well-perfused A549 tumors.

AB - Purpose: Blood oxygen level dependent (BOLD) MRI based on R2* measurements can provide insights into tumor vascular oxygenation. However, measurements are susceptible to blood flow, which may vary accompanying a hyperoxic gas challenge. We investigated flow sensitivity by comparing R2* measurements with and without flow suppression (fs) in 2 orthotopic lung xenograft tumor models. Methods: H460 (n = 20) and A549 (n = 20) human lung tumor xenografts were induced by surgical implantation of cancer cells in the right lung of nude rats. MRI was performed at 4.7T after tumors reached 5 to 8 mm in diameter. A multiecho gradient echo MRI sequence was acquired with and without spatial saturation bands on each side of the imaging plane to evaluate the effect of flow on R2*. fs and non-fs R2* MRI measurements were interleaved during an oxygen breathing challenge (from air to 100% O2). T2*-weighted signal intensity changes (ΔSI(%)) and R2* measurements were obtained for regions of interest and on a voxel-by-voxel basis and discrepancies quantified with Bland–Altman analysis. Results: Flow suppression affected ΔSI(%) and R2* measurements in each tumor model. Average discrepancy and limits of agreement from Bland–Altman analyses revealed greater flow-related bias in A549 than H460. Conclusion: The effect of flow on R2*, and hence BOLD, was tumor model dependent with measurements being more sensitive in well-perfused A549 tumors.

KW - blood oxygen level dependent (BOLD)

KW - flow-suppression

KW - hypoxia

KW - lung cancer

KW - oxygen-sensitive MRI

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