The effect of glutamate on ghrelin release in mice

Rakhi Chacrabati, Zhi Gong, Chika Ikenoya, Daisuke Kondo, Jeffrey M. Zigman, Takafumi Sakai, Ichiro Sakata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.

Original languageEnglish (US)
Pages (from-to)320-327
Number of pages8
JournalCell Biology International
Volume41
Issue number3
DOIs
StatePublished - Mar 1 2017

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Ghrelin
Glutamic Acid
Extracellular Signal-Regulated MAP Kinases
G-Protein-Coupled Receptors
Cell Line
Small Interfering RNA
Stomach
Eating

Keywords

  • CREB2
  • extracellular signal-regulated kinase (ERK)
  • ghrelin
  • glutamate
  • GPRC5B

ASJC Scopus subject areas

  • Cell Biology

Cite this

Chacrabati, R., Gong, Z., Ikenoya, C., Kondo, D., Zigman, J. M., Sakai, T., & Sakata, I. (2017). The effect of glutamate on ghrelin release in mice. Cell Biology International, 41(3), 320-327. https://doi.org/10.1002/cbin.10728

The effect of glutamate on ghrelin release in mice. / Chacrabati, Rakhi; Gong, Zhi; Ikenoya, Chika; Kondo, Daisuke; Zigman, Jeffrey M.; Sakai, Takafumi; Sakata, Ichiro.

In: Cell Biology International, Vol. 41, No. 3, 01.03.2017, p. 320-327.

Research output: Contribution to journalArticle

Chacrabati, R, Gong, Z, Ikenoya, C, Kondo, D, Zigman, JM, Sakai, T & Sakata, I 2017, 'The effect of glutamate on ghrelin release in mice', Cell Biology International, vol. 41, no. 3, pp. 320-327. https://doi.org/10.1002/cbin.10728
Chacrabati R, Gong Z, Ikenoya C, Kondo D, Zigman JM, Sakai T et al. The effect of glutamate on ghrelin release in mice. Cell Biology International. 2017 Mar 1;41(3):320-327. https://doi.org/10.1002/cbin.10728
Chacrabati, Rakhi ; Gong, Zhi ; Ikenoya, Chika ; Kondo, Daisuke ; Zigman, Jeffrey M. ; Sakai, Takafumi ; Sakata, Ichiro. / The effect of glutamate on ghrelin release in mice. In: Cell Biology International. 2017 ; Vol. 41, No. 3. pp. 320-327.
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