The effect of insulin and glucose on fructose-2,6-P2 in hepatocytes

Carolyn Sue Richards, Kosaku Uyeda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effect of insulin and glucose on fructose-2,6-P2 turnover has been examined using isolated rat hepatocytes. Insulin (>10-10M) increases the fructose-2,6-P2 level in hepatocytes from fasted rats, or those pretreated with glucagon (<10-10M) but not those from fed rats. Glucose (>10mM) also increases the fructose-2,6-P2 and hexose-P levels in hepatocytes from starved rats, and its effect appears to be greater than that of insulin. Furthermore, insulin increases fructose-6-P,2-kinase activity and decreases cAMP levels in hepatocytes pretreated with glucagon, and it increases hexose-P concentration in hepatocytes from fasted rats. These results suggest that insulin antagonizes glucagon action by increasing fructose-6-P,2-kinase, decreasing cyclic AMP levels, and increasing hexose-P levels, resulting in increased concentrations of fructose-2,6-P2.

Original languageEnglish (US)
Pages (from-to)394-401
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume109
Issue number2
DOIs
StatePublished - Nov 30 1982

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Fructose
Hepatocytes
Hexoses
Insulin
Rats
Glucose
Phosphofructokinase-2
Glucagon
Cyclic AMP

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The effect of insulin and glucose on fructose-2,6-P2 in hepatocytes. / Richards, Carolyn Sue; Uyeda, Kosaku.

In: Biochemical and Biophysical Research Communications, Vol. 109, No. 2, 30.11.1982, p. 394-401.

Research output: Contribution to journalArticle

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