The effect of insulin and insulin-like growth factors on the expression of steroidogenic enzymes in a human ovarian thecal-like tumor cell model

E. A. McGee, C. Sawetawan, I. Bird, W. E. Rainey, B. R. Carr

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: To determine the effects of insulin and insulin-like growth factors (IGF-I and IGF-II) on steroidogenesis and steroidogenic enzyme expression in a human ovarian thecal-like tumor cell culture model system. Design: Human ovarian thecal-like tumor cells treated with forskolin and insulin IGF-I or IGF-II were evaluated for media accumulation of P and androstenedione (A) as well as 3β-hydroxysteroid dehydrogenase (3βHSD) and cytochrome P450 17α-hydroxylase (P450c17) enzyme activity. Northern analysis of cytochrome P450 side chain cleavage (P450scc), P450c17, and 3βHSD messenger RNA (mRNA) also was performed. Results: Basal hormone secretion, enzyme activity, and mRNA levels were not affected by treatment with insulin or the IGFs. Forskolin treatment stimulated steroid production, enzyme activity, and mRNA content. Forskolin-stimulated P secretion was augmented 30% by treatment with insulin and IGFs, whereas 3βHSD activity was augmented twofold to threefold. Forskolin stimulated A and P450c17 activity were enhanced by treatment with insulin and the [GFs. In forskolin-treated cells, P450c17 and P450scc mRNA levels were not affected by insulin (100 nM) or IGF (10 nM) treatment; however, 3βHSD mRNA levels were augmented by treatment with insulin and IGFs. Conclusions: We observed that forskolin-stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17, and 3βHSD activity, as well as mRNA content for P450scc, 3βHSD, and P450c17. Insulin and the IGFs augmented forskolin-stimulated production of P and the expression of 3βHSD, with little effect on A production, P450scc, or P450c17 expression.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalFertility and Sterility
Volume65
Issue number1
StatePublished - 1996

Fingerprint

3-Hydroxysteroid Dehydrogenases
Somatomedins
Colforsin
Insulin
Enzymes
Messenger RNA
Neoplasms
Insulin-Like Growth Factor I
Insulin-Like Growth Factor II
Cytochrome P-450 Enzyme System
Androstenedione
Mixed Function Oxygenases
Cell Culture Techniques
Steroids
Hormones

Keywords

  • 3βHSD
  • insulin
  • insulin-like growth factors
  • Ovary
  • P450c17
  • steroidogenesis
  • theca

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

The effect of insulin and insulin-like growth factors on the expression of steroidogenic enzymes in a human ovarian thecal-like tumor cell model. / McGee, E. A.; Sawetawan, C.; Bird, I.; Rainey, W. E.; Carr, B. R.

In: Fertility and Sterility, Vol. 65, No. 1, 1996, p. 87-93.

Research output: Contribution to journalArticle

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abstract = "Objective: To determine the effects of insulin and insulin-like growth factors (IGF-I and IGF-II) on steroidogenesis and steroidogenic enzyme expression in a human ovarian thecal-like tumor cell culture model system. Design: Human ovarian thecal-like tumor cells treated with forskolin and insulin IGF-I or IGF-II were evaluated for media accumulation of P and androstenedione (A) as well as 3β-hydroxysteroid dehydrogenase (3βHSD) and cytochrome P450 17α-hydroxylase (P450c17) enzyme activity. Northern analysis of cytochrome P450 side chain cleavage (P450scc), P450c17, and 3βHSD messenger RNA (mRNA) also was performed. Results: Basal hormone secretion, enzyme activity, and mRNA levels were not affected by treatment with insulin or the IGFs. Forskolin treatment stimulated steroid production, enzyme activity, and mRNA content. Forskolin-stimulated P secretion was augmented 30{\%} by treatment with insulin and IGFs, whereas 3βHSD activity was augmented twofold to threefold. Forskolin stimulated A and P450c17 activity were enhanced by treatment with insulin and the [GFs. In forskolin-treated cells, P450c17 and P450scc mRNA levels were not affected by insulin (100 nM) or IGF (10 nM) treatment; however, 3βHSD mRNA levels were augmented by treatment with insulin and IGFs. Conclusions: We observed that forskolin-stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17, and 3βHSD activity, as well as mRNA content for P450scc, 3βHSD, and P450c17. Insulin and the IGFs augmented forskolin-stimulated production of P and the expression of 3βHSD, with little effect on A production, P450scc, or P450c17 expression.",
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T1 - The effect of insulin and insulin-like growth factors on the expression of steroidogenic enzymes in a human ovarian thecal-like tumor cell model

AU - McGee, E. A.

AU - Sawetawan, C.

AU - Bird, I.

AU - Rainey, W. E.

AU - Carr, B. R.

PY - 1996

Y1 - 1996

N2 - Objective: To determine the effects of insulin and insulin-like growth factors (IGF-I and IGF-II) on steroidogenesis and steroidogenic enzyme expression in a human ovarian thecal-like tumor cell culture model system. Design: Human ovarian thecal-like tumor cells treated with forskolin and insulin IGF-I or IGF-II were evaluated for media accumulation of P and androstenedione (A) as well as 3β-hydroxysteroid dehydrogenase (3βHSD) and cytochrome P450 17α-hydroxylase (P450c17) enzyme activity. Northern analysis of cytochrome P450 side chain cleavage (P450scc), P450c17, and 3βHSD messenger RNA (mRNA) also was performed. Results: Basal hormone secretion, enzyme activity, and mRNA levels were not affected by treatment with insulin or the IGFs. Forskolin treatment stimulated steroid production, enzyme activity, and mRNA content. Forskolin-stimulated P secretion was augmented 30% by treatment with insulin and IGFs, whereas 3βHSD activity was augmented twofold to threefold. Forskolin stimulated A and P450c17 activity were enhanced by treatment with insulin and the [GFs. In forskolin-treated cells, P450c17 and P450scc mRNA levels were not affected by insulin (100 nM) or IGF (10 nM) treatment; however, 3βHSD mRNA levels were augmented by treatment with insulin and IGFs. Conclusions: We observed that forskolin-stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17, and 3βHSD activity, as well as mRNA content for P450scc, 3βHSD, and P450c17. Insulin and the IGFs augmented forskolin-stimulated production of P and the expression of 3βHSD, with little effect on A production, P450scc, or P450c17 expression.

AB - Objective: To determine the effects of insulin and insulin-like growth factors (IGF-I and IGF-II) on steroidogenesis and steroidogenic enzyme expression in a human ovarian thecal-like tumor cell culture model system. Design: Human ovarian thecal-like tumor cells treated with forskolin and insulin IGF-I or IGF-II were evaluated for media accumulation of P and androstenedione (A) as well as 3β-hydroxysteroid dehydrogenase (3βHSD) and cytochrome P450 17α-hydroxylase (P450c17) enzyme activity. Northern analysis of cytochrome P450 side chain cleavage (P450scc), P450c17, and 3βHSD messenger RNA (mRNA) also was performed. Results: Basal hormone secretion, enzyme activity, and mRNA levels were not affected by treatment with insulin or the IGFs. Forskolin treatment stimulated steroid production, enzyme activity, and mRNA content. Forskolin-stimulated P secretion was augmented 30% by treatment with insulin and IGFs, whereas 3βHSD activity was augmented twofold to threefold. Forskolin stimulated A and P450c17 activity were enhanced by treatment with insulin and the [GFs. In forskolin-treated cells, P450c17 and P450scc mRNA levels were not affected by insulin (100 nM) or IGF (10 nM) treatment; however, 3βHSD mRNA levels were augmented by treatment with insulin and IGFs. Conclusions: We observed that forskolin-stimulated human ovarian thecal-like tumor cell steroidogenesis, P450c17, and 3βHSD activity, as well as mRNA content for P450scc, 3βHSD, and P450c17. Insulin and the IGFs augmented forskolin-stimulated production of P and the expression of 3βHSD, with little effect on A production, P450scc, or P450c17 expression.

KW - 3βHSD

KW - insulin

KW - insulin-like growth factors

KW - Ovary

KW - P450c17

KW - steroidogenesis

KW - theca

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