TY - JOUR
T1 - The effect of osmotic stress on the production of nukacin ISK-1 from Staphylococcus warneri ISK-1
AU - National Cardiovascular Network
AU - Peterson, Eric D.
AU - Lansky, Alexandra J.
AU - Anstrom, Kevin J.
AU - Muhlbaier, Lawrence H.
AU - Popma, Jeffrey J.
AU - Satler, Lowell F.
AU - Lanzilotta, Michael J.
PY - 2001
Y1 - 2001
N2 - The effects of several additives on the production of a lantibiotic, nukacin ISK-1, from Staphylococcus warneri ISK-1, in batch fermentation were studied. NaCl, KCl and sorbitol stimulated nukacin ISK-1 production. The addition of 1.4 M NaCl increased nukacin ISK-1 activity 1.5-fold over the control, while cell growth and glucose consumption were inhibited. Nukacin ISK-1 production increased with increasing osmolarity of the medium up to about 3 osmol/kg; however, further increases in osmolarity diminished productivity, irrespective of the kind of additive. Northern blot analysis showed that transcription of the nukacin ISK-1 structural gene (nukA) was activated in the presence of 1.4 M NaCl. These data indicate that the stimulation effect was due to osmotic stress, which acted, at least in part, at the transcriptional level on the nukA gene.
AB - The effects of several additives on the production of a lantibiotic, nukacin ISK-1, from Staphylococcus warneri ISK-1, in batch fermentation were studied. NaCl, KCl and sorbitol stimulated nukacin ISK-1 production. The addition of 1.4 M NaCl increased nukacin ISK-1 activity 1.5-fold over the control, while cell growth and glucose consumption were inhibited. Nukacin ISK-1 production increased with increasing osmolarity of the medium up to about 3 osmol/kg; however, further increases in osmolarity diminished productivity, irrespective of the kind of additive. Northern blot analysis showed that transcription of the nukacin ISK-1 structural gene (nukA) was activated in the presence of 1.4 M NaCl. These data indicate that the stimulation effect was due to osmotic stress, which acted, at least in part, at the transcriptional level on the nukA gene.
UR - http://www.scopus.com/inward/record.url?scp=0034882625&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034882625&partnerID=8YFLogxK
U2 - 10.1007/s002530100669
DO - 10.1007/s002530100669
M3 - Article
C2 - 11549026
AN - SCOPUS:0034882625
SN - 0175-7598
VL - 56
SP - 496
EP - 501
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 3-4
ER -