The effect of polypeptide hormones on lipid monolayers. II. The effect of insulin analogues, vasopressin, oxytocin, thyrocalcitonin, adrenocorticotropin, and 3′,5′-cyclic AMP on the uptake of Ca2+ by monomolecular films of monooctadecyl phosphate

Marian S. Kafka, Charles Y C Pak

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

1. 1. Like insulin, some analogues of insulin inhibited the adsorption of Ca2+ at a monooctadecyl phosphate monolayer; the inhibition was, in all cases, less than the inhibition by intact insulin. 2. 2. Facilitation of release of Ca2+ from the monolayer by insulin required that the insulin molecule be intact, as release was facilitated only very slightly when des-A1-glycine-des-B1-phenylalanine1-insulin or insulin B-chain-S-sulfonate replaced insulin, and did not occur at all in the absence of alanine in position B30. 3. 3. Vasopressin and oxytocin inhibited Ca2+ adsorption. In addition, vasopressin facilitated Ca2+ release. 4. 4. Thyrocalcitonin profoundly inhibited Ca2+ adsorption but facilitated only slightly its release. 5. 5. 3′,5′-cyclic AMP facilitated slightly the adsorption of Ca2+. 6. 6. It is suggested that the biphasic action of insulin and vasopressin on Ca2+ uptake depends upon the concurrence of a 6-membered disulfide ring at or near the N-terminus of the molecule, and a proline followed by a positively charged amino acid (arginine or lysine) in the two positions preceding the C-terminal amino acid.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalBBA - Biomembranes
Volume193
Issue number1
StatePublished - Oct 14 1969

Fingerprint

Peptide Hormones
Calcitonin
Oxytocin
Vasopressins
Cyclic AMP
Adrenocorticotropic Hormone
Monolayers
Phosphates
Insulin
Lipids
Adsorption
Biphasic Insulins
Amino Acids
Molecules
Proline
Disulfides
Alanine
Glycine
Lysine
Arginine

ASJC Scopus subject areas

  • Cell Biology
  • Biophysics
  • Biochemistry
  • Medicine(all)

Cite this

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abstract = "1. 1. Like insulin, some analogues of insulin inhibited the adsorption of Ca2+ at a monooctadecyl phosphate monolayer; the inhibition was, in all cases, less than the inhibition by intact insulin. 2. 2. Facilitation of release of Ca2+ from the monolayer by insulin required that the insulin molecule be intact, as release was facilitated only very slightly when des-A1-glycine-des-B1-phenylalanine1-insulin or insulin B-chain-S-sulfonate replaced insulin, and did not occur at all in the absence of alanine in position B30. 3. 3. Vasopressin and oxytocin inhibited Ca2+ adsorption. In addition, vasopressin facilitated Ca2+ release. 4. 4. Thyrocalcitonin profoundly inhibited Ca2+ adsorption but facilitated only slightly its release. 5. 5. 3′,5′-cyclic AMP facilitated slightly the adsorption of Ca2+. 6. 6. It is suggested that the biphasic action of insulin and vasopressin on Ca2+ uptake depends upon the concurrence of a 6-membered disulfide ring at or near the N-terminus of the molecule, and a proline followed by a positively charged amino acid (arginine or lysine) in the two positions preceding the C-terminal amino acid.",
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N2 - 1. 1. Like insulin, some analogues of insulin inhibited the adsorption of Ca2+ at a monooctadecyl phosphate monolayer; the inhibition was, in all cases, less than the inhibition by intact insulin. 2. 2. Facilitation of release of Ca2+ from the monolayer by insulin required that the insulin molecule be intact, as release was facilitated only very slightly when des-A1-glycine-des-B1-phenylalanine1-insulin or insulin B-chain-S-sulfonate replaced insulin, and did not occur at all in the absence of alanine in position B30. 3. 3. Vasopressin and oxytocin inhibited Ca2+ adsorption. In addition, vasopressin facilitated Ca2+ release. 4. 4. Thyrocalcitonin profoundly inhibited Ca2+ adsorption but facilitated only slightly its release. 5. 5. 3′,5′-cyclic AMP facilitated slightly the adsorption of Ca2+. 6. 6. It is suggested that the biphasic action of insulin and vasopressin on Ca2+ uptake depends upon the concurrence of a 6-membered disulfide ring at or near the N-terminus of the molecule, and a proline followed by a positively charged amino acid (arginine or lysine) in the two positions preceding the C-terminal amino acid.

AB - 1. 1. Like insulin, some analogues of insulin inhibited the adsorption of Ca2+ at a monooctadecyl phosphate monolayer; the inhibition was, in all cases, less than the inhibition by intact insulin. 2. 2. Facilitation of release of Ca2+ from the monolayer by insulin required that the insulin molecule be intact, as release was facilitated only very slightly when des-A1-glycine-des-B1-phenylalanine1-insulin or insulin B-chain-S-sulfonate replaced insulin, and did not occur at all in the absence of alanine in position B30. 3. 3. Vasopressin and oxytocin inhibited Ca2+ adsorption. In addition, vasopressin facilitated Ca2+ release. 4. 4. Thyrocalcitonin profoundly inhibited Ca2+ adsorption but facilitated only slightly its release. 5. 5. 3′,5′-cyclic AMP facilitated slightly the adsorption of Ca2+. 6. 6. It is suggested that the biphasic action of insulin and vasopressin on Ca2+ uptake depends upon the concurrence of a 6-membered disulfide ring at or near the N-terminus of the molecule, and a proline followed by a positively charged amino acid (arginine or lysine) in the two positions preceding the C-terminal amino acid.

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