The effects of elastic fiber protein insufficiency and treatment on the modulus of arterial smooth muscle cells

M. Gabriela Espinosa, William S. Gardner, Lisa Bennett, Bradley A. Sather, Hiromi Yanagisawa, Jessica E. Wagenseil

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Abstract

Elastic fibers are critical for the mechanical function of the large arteries. Mechanical effects of elastic fiber protein deficiency have been investigated in whole arteries, but not in isolated smooth muscle cells (SMCs). The elastic moduli of SMCs from elastin (Eln-/-) and fibulin-4 (Fbln4-/-) knockout mice were measured using atomic force microscopy. Compared to control SMCs, the modulus of Eln-/-SMCs is reduced by 40%, but is unchanged in Fbln4-/-SMCs. The Eln-/-SMC modulus is rescued by soluble or α elastin treatment. Altered gene expression, specifically of calponin, suggests that SMC phenotypic modulation may be responsible for the modulus changes.

Original languageEnglish (US)
Article number021030
JournalJournal of Biomechanical Engineering
Volume136
Issue number2
DOIs
StatePublished - Feb 2014

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ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Cite this

Gabriela Espinosa, M., Gardner, W. S., Bennett, L., Sather, B. A., Yanagisawa, H., & Wagenseil, J. E. (2014). The effects of elastic fiber protein insufficiency and treatment on the modulus of arterial smooth muscle cells. Journal of Biomechanical Engineering, 136(2), [021030]. https://doi.org/10.1115/1.4026203