The effects of galectin-3 depletion apheresis on induced skin inflammation in a porcine model

Nalu Navarro-Alvarez, Beatriz Goncalves, Alec R. Andrews, Zhaohui Wang, Zhirui Wang, Edward Harrington, Jigesh Shah, David H. Sachs, Isaac Eliaz, Christene A. Huang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Galectin-3 (Gal-3), a β-galactoside-binding lectin that is expressed in mammalian cells, is known to modulate several biological functions such as cell-cell adhesion, macrophage activation, angiogenesis, metastasis, and fibrosis. The goal of this study was to evaluate the ability of Gal-3 depletion apheresis using an adsorption column with immobilized anti-Gal-3-antibody to reduce inflammation induced by Complete Freund's Adjuvant injection in a skin inflammation porcine model. Here, we report that plasma perfusion by apheresis through a Gal-3 binding immuno-affinity column reduces plasma Gal-3 levels to below limits of quantitative detection, and results in significant decrease in skin inflammation, including degree and duration of inflammatory lesions. Human plasma was tested ex vivo and found to be efficiently depleted using the anti-Gal-3 affinity column. This study demonstrates the potential of Gal-3 depletion apheresis as a therapeutic method for inflammation-mediated disease, supporting continued research in this area for clinical application.

Original languageEnglish (US)
Pages (from-to)486-493
Number of pages8
JournalJournal of Clinical Apheresis
Volume33
Issue number4
DOIs
StatePublished - Aug 2018
Externally publishedYes

Keywords

  • complete Freund's adjuvant
  • lesion induration
  • plasmapheresis
  • therapeutic-apheresis

ASJC Scopus subject areas

  • Hematology

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