The effects of moxifloxacin ophthalmic solution 0.5% or gatifloxacin ophthalmic solution 0.3% treatment on corneal wound healing in pigmented rabbits following anterior keratectomy

K. Keven Williams, Mitchell D. McCartney, Rebecca L. Rice, Martin B. Wax, Joseph W. Hiddemen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose These studies examined corneal healing rates, Type-IV collagen and zonula occludens membrane-associated protein (ZO-1) expression, as well as aqueous PGE2 and IL-1β concentrations in pigmented rabbits treated with either moxifloxacin 0.5%, gatifloxacin 0.3% or BSS® following anterior keratectomy. Methods Anterior keratectomy surgery was followed by topical administration with commercial ophthalmic formulations of either moxifloxacin or gatifloxacin or BSS® (TID for 96 h). Images of the fluorescein-stained healing corneas were analyzed for wound area. At 48 or 96 h following surgery, aqueous humor samples were collected and analyzed for the inflammatory mediators PGE2 and IL-1β using an ELISA. The corneas were subsequently evaluated using both scanning and transmission electron microscopy. In a second parallel study, corneas were evaluated at both 48 and 96 h for Type-IV collagen and ZO-1 expression using immunohistochemistry. Results Fluorescein-stained corneal images at 96 h postsurgery demonstrated that 90% ± 8% re-epithelialization for moxifloxacin, 81% ± 14% for gatifloxacin, and 88 ± 6% for BSS ® (P > 0.05). PGE2levels in the aqueous humor of fluoroquinolone treated eyes were reduced at 48 h compared to BSS ® treated eyes. IL-1β was undetectable in all samples. No differences in Type-IV collagen or ZO-1 expression were observed between any treatment groups. There were no differences between groups in histological appearance or in ultrastructural healing processes. Conclusions These studies demonstrated that the commercial ophthalmic formulations of moxifloxacin and gatifloxacin were similar to each other in their effects on the levels of aqueous humor PGE2 and rates of corneal wound re-epithelialization.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalVeterinary Ophthalmology
Volume11
Issue number5
DOIs
StatePublished - Sep 2008

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Ophthalmic Solutions
Re-Epithelialization
tissue repair
Wound Healing
Collagen Type IV
Aqueous Humor
interleukin-1
cornea
eyes
rabbits
Interleukin-1
Dinoprostone
Cornea
Rabbits
collagen
fluorescein
Fluorescein
animal injuries
Zonula Occludens Proteins
surgery

Keywords

  • Antibiotic
  • Corneal wound healing
  • Gatifloxacin
  • IL-1β
  • Moxifloxacin
  • PGE
  • Type-IV collagen

ASJC Scopus subject areas

  • Ophthalmology
  • veterinary(all)

Cite this

The effects of moxifloxacin ophthalmic solution 0.5% or gatifloxacin ophthalmic solution 0.3% treatment on corneal wound healing in pigmented rabbits following anterior keratectomy. / Williams, K. Keven; McCartney, Mitchell D.; Rice, Rebecca L.; Wax, Martin B.; Hiddemen, Joseph W.

In: Veterinary Ophthalmology, Vol. 11, No. 5, 09.2008, p. 327-334.

Research output: Contribution to journalArticle

Williams, K. Keven ; McCartney, Mitchell D. ; Rice, Rebecca L. ; Wax, Martin B. ; Hiddemen, Joseph W. / The effects of moxifloxacin ophthalmic solution 0.5% or gatifloxacin ophthalmic solution 0.3% treatment on corneal wound healing in pigmented rabbits following anterior keratectomy. In: Veterinary Ophthalmology. 2008 ; Vol. 11, No. 5. pp. 327-334.
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abstract = "Purpose These studies examined corneal healing rates, Type-IV collagen and zonula occludens membrane-associated protein (ZO-1) expression, as well as aqueous PGE2 and IL-1β concentrations in pigmented rabbits treated with either moxifloxacin 0.5{\%}, gatifloxacin 0.3{\%} or BSS{\circledR} following anterior keratectomy. Methods Anterior keratectomy surgery was followed by topical administration with commercial ophthalmic formulations of either moxifloxacin or gatifloxacin or BSS{\circledR} (TID for 96 h). Images of the fluorescein-stained healing corneas were analyzed for wound area. At 48 or 96 h following surgery, aqueous humor samples were collected and analyzed for the inflammatory mediators PGE2 and IL-1β using an ELISA. The corneas were subsequently evaluated using both scanning and transmission electron microscopy. In a second parallel study, corneas were evaluated at both 48 and 96 h for Type-IV collagen and ZO-1 expression using immunohistochemistry. Results Fluorescein-stained corneal images at 96 h postsurgery demonstrated that 90{\%} ± 8{\%} re-epithelialization for moxifloxacin, 81{\%} ± 14{\%} for gatifloxacin, and 88 ± 6{\%} for BSS {\circledR} (P > 0.05). PGE2levels in the aqueous humor of fluoroquinolone treated eyes were reduced at 48 h compared to BSS {\circledR} treated eyes. IL-1β was undetectable in all samples. No differences in Type-IV collagen or ZO-1 expression were observed between any treatment groups. There were no differences between groups in histological appearance or in ultrastructural healing processes. Conclusions These studies demonstrated that the commercial ophthalmic formulations of moxifloxacin and gatifloxacin were similar to each other in their effects on the levels of aqueous humor PGE2 and rates of corneal wound re-epithelialization.",
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AU - McCartney, Mitchell D.

AU - Rice, Rebecca L.

AU - Wax, Martin B.

AU - Hiddemen, Joseph W.

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AB - Purpose These studies examined corneal healing rates, Type-IV collagen and zonula occludens membrane-associated protein (ZO-1) expression, as well as aqueous PGE2 and IL-1β concentrations in pigmented rabbits treated with either moxifloxacin 0.5%, gatifloxacin 0.3% or BSS® following anterior keratectomy. Methods Anterior keratectomy surgery was followed by topical administration with commercial ophthalmic formulations of either moxifloxacin or gatifloxacin or BSS® (TID for 96 h). Images of the fluorescein-stained healing corneas were analyzed for wound area. At 48 or 96 h following surgery, aqueous humor samples were collected and analyzed for the inflammatory mediators PGE2 and IL-1β using an ELISA. The corneas were subsequently evaluated using both scanning and transmission electron microscopy. In a second parallel study, corneas were evaluated at both 48 and 96 h for Type-IV collagen and ZO-1 expression using immunohistochemistry. Results Fluorescein-stained corneal images at 96 h postsurgery demonstrated that 90% ± 8% re-epithelialization for moxifloxacin, 81% ± 14% for gatifloxacin, and 88 ± 6% for BSS ® (P > 0.05). PGE2levels in the aqueous humor of fluoroquinolone treated eyes were reduced at 48 h compared to BSS ® treated eyes. IL-1β was undetectable in all samples. No differences in Type-IV collagen or ZO-1 expression were observed between any treatment groups. There were no differences between groups in histological appearance or in ultrastructural healing processes. Conclusions These studies demonstrated that the commercial ophthalmic formulations of moxifloxacin and gatifloxacin were similar to each other in their effects on the levels of aqueous humor PGE2 and rates of corneal wound re-epithelialization.

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