The endogenous lectin RL-29 is transynaptically induced in dorsal horn neurons following peripheral neuropathy in the rat

Adrian A. Cameron, Patrick M. Dougherty, Christopher J. Garrison, William D. Willis, Susan M. Carlton

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Neurons containing immunoreactivity to the endogenous lactose-binding lectin, RL-29, were examined in the L4 segment of the spinal cord of rats with an experimental neuropathy. The cells appeared by 5 days and were also present at 14 and 28 days postoperatively. All neurons were found in the dorsal horn ipsilateral to the injury. The neurons were multipolar and the reaction product revealed the morphology of the primary and secondary dendrites and some axons. Most of the neurons were located in the reticulated region of the dorsal horn, corresponding to Rexed's lamina V. In 14-day neuropathy animals treated with the NMDA-receptor antagonist MK-801, the number of RL-29 cell profiles observed was significantly reduced. Double labeling experiments revealed that spinothalamic tract neurons did not contain RL-29. The results suggest that the neuropathic injury produces a long term, transynaptic change in a subpopulation of dorsal horn neurons, that is mediated by excitatory amino acid transmitters acting at NMDA receptors.

Original languageEnglish (US)
Pages (from-to)64-71
Number of pages8
JournalBrain Research
Volume620
Issue number1
DOIs
StatePublished - Aug 20 1993

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Posterior Horn Cells
Peripheral Nervous System Diseases
Lectins
Neurons
N-Methyl-D-Aspartate Receptors
Spinothalamic Tracts
Excitatory Amino Acids
Dizocilpine Maleate
Wounds and Injuries
Lactose
Dendrites
Axons
Spinal Cord

Keywords

  • Immediate early gene
  • NMDA antagonist
  • Sciatic nerve
  • Transynaptic induction

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

The endogenous lectin RL-29 is transynaptically induced in dorsal horn neurons following peripheral neuropathy in the rat. / Cameron, Adrian A.; Dougherty, Patrick M.; Garrison, Christopher J.; Willis, William D.; Carlton, Susan M.

In: Brain Research, Vol. 620, No. 1, 20.08.1993, p. 64-71.

Research output: Contribution to journalArticle

Cameron, Adrian A. ; Dougherty, Patrick M. ; Garrison, Christopher J. ; Willis, William D. ; Carlton, Susan M. / The endogenous lectin RL-29 is transynaptically induced in dorsal horn neurons following peripheral neuropathy in the rat. In: Brain Research. 1993 ; Vol. 620, No. 1. pp. 64-71.
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