The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2

Erin C. Zook, Kevin Ramirez, Xiaohuan Guo, Grant van der Voort, Mikael Sigvardsson, Eric C. Svensson, Yang Xin Fu, Barbara L. Kee

Research output: Contribution to journalArticle

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Abstract

Group 2 innate lymphoid cells (ILC2s) are a subset of ILCs that play a protective role in the response to helminth infection, but they also contribute to allergic lung inflammation. Here, we report that the deletion of the ETS1 transcription factor in lymphoid cells resulted in a loss of ILC2s in the bone marrow and lymph nodes and that ETS1 promotes the fitness of the common progenitor of all ILCs. ETS1-deficient ILC2 progenitors failed to up-regulate messenger RNA for the E protein transcription factor inhibitor ID2, a critical factor for ILCs, and these cells were unable to expand in cytokine-driven in vitro cultures. In vivo, ETS1 was required for the IL-33-induced accumulation of lung ILC2s and for the production of the T helper type 2 cytokines IL-5 and IL-13. IL-25 also failed to elicit an expansion of inflammatory ILC2s when these cells lacked ETS1. Our data reveal ETS1 as a critical regulator of ILC2 expansion and cytokine production and implicate ETS1 in the regulation of Id2 at the inception of ILC2 development.

Original languageEnglish (US)
Pages (from-to)687-696
Number of pages10
JournalJournal of Experimental Medicine
Volume213
Issue number5
DOIs
StatePublished - Jan 1 2016

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Transcription Factors
Cytokines
Lymphocytes
Interleukin-13
Helminths
Interleukin-5
Pneumonia
Up-Regulation
Lymph Nodes
Bone Marrow
Lung
Messenger RNA
Infection
Proteins
Interleukin-33
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Zook, E. C., Ramirez, K., Guo, X., van der Voort, G., Sigvardsson, M., Svensson, E. C., ... Kee, B. L. (2016). The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2. Journal of Experimental Medicine, 213(5), 687-696. https://doi.org/10.1084/jem.20150851

The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2. / Zook, Erin C.; Ramirez, Kevin; Guo, Xiaohuan; van der Voort, Grant; Sigvardsson, Mikael; Svensson, Eric C.; Fu, Yang Xin; Kee, Barbara L.

In: Journal of Experimental Medicine, Vol. 213, No. 5, 01.01.2016, p. 687-696.

Research output: Contribution to journalArticle

Zook, EC, Ramirez, K, Guo, X, van der Voort, G, Sigvardsson, M, Svensson, EC, Fu, YX & Kee, BL 2016, 'The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2', Journal of Experimental Medicine, vol. 213, no. 5, pp. 687-696. https://doi.org/10.1084/jem.20150851
Zook, Erin C. ; Ramirez, Kevin ; Guo, Xiaohuan ; van der Voort, Grant ; Sigvardsson, Mikael ; Svensson, Eric C. ; Fu, Yang Xin ; Kee, Barbara L. / The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2. In: Journal of Experimental Medicine. 2016 ; Vol. 213, No. 5. pp. 687-696.
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