The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells

Melissa A. Altura, Elizabeth A C Heath-Heckman, Amani Gillette, Natacha Kremer, Anne Marie Krachler, Caitlin Brennan, Edward G. Ruby, Kim Orth, Margaret J. Mcfall-Ngai

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Summary: We studied the Euprymna scolopes-Vibrio fischeri symbiosis to characterize, in vivo and in real time, the transition between the bacterial partner's free-living and symbiotic life styles. Previous studies using high inocula demonstrated that environmental V.fischeri cells aggregate during a 3h period in host-shed mucus along the light organ's superficial ciliated epithelia. Under lower inoculum conditions, similar to the levels of symbiont cells in the environment, this interaction induces haemocyte trafficking into these tissues. Here, in experiments simulating natural conditions, microscopy revealed that at 3h following first exposure, only ~5 V.fischeri cells aggregated on the organ surface. These cells associated with host cilia and induced haemocyte trafficking. Symbiont viability was essential and mutants defective in symbiosis initiation and/or production of certain surface features, including the Mam7 protein, which is implicated in host cell attachment of V.cholerae, associated normally with host cilia. Studies with exopolysaccharide mutants, which are defective in aggregation, suggest a two-step process of V.fischeri cell engagement: association with host cilia followed by aggregation, i.e. host cell-symbiont interaction with subsequent symbiont-symbiont cell interaction. Taken together, these data provide a new model of early partner engagement, a complex model of host-symbiont interaction with exquisite sensitivity.

Original languageEnglish (US)
Pages (from-to)2937-2950
Number of pages14
JournalEnvironmental Microbiology
Volume15
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Aliivibrio fischeri
Vibrio fischeri
Symbiosis
symbiont
symbiosis
symbionts
Cilia
trafficking
cilia
Hemocytes
cells
host-symbiont interaction
Cell Communication
hemocytes
cholera
inoculum
mucus
Cholera
mutants
Mucus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Altura, M. A., Heath-Heckman, E. A. C., Gillette, A., Kremer, N., Krachler, A. M., Brennan, C., ... Mcfall-Ngai, M. J. (2013). The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells. Environmental Microbiology, 15(11), 2937-2950. https://doi.org/10.1111/1462-2920.12179

The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells. / Altura, Melissa A.; Heath-Heckman, Elizabeth A C; Gillette, Amani; Kremer, Natacha; Krachler, Anne Marie; Brennan, Caitlin; Ruby, Edward G.; Orth, Kim; Mcfall-Ngai, Margaret J.

In: Environmental Microbiology, Vol. 15, No. 11, 11.2013, p. 2937-2950.

Research output: Contribution to journalArticle

Altura, MA, Heath-Heckman, EAC, Gillette, A, Kremer, N, Krachler, AM, Brennan, C, Ruby, EG, Orth, K & Mcfall-Ngai, MJ 2013, 'The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells', Environmental Microbiology, vol. 15, no. 11, pp. 2937-2950. https://doi.org/10.1111/1462-2920.12179
Altura, Melissa A. ; Heath-Heckman, Elizabeth A C ; Gillette, Amani ; Kremer, Natacha ; Krachler, Anne Marie ; Brennan, Caitlin ; Ruby, Edward G. ; Orth, Kim ; Mcfall-Ngai, Margaret J. / The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells. In: Environmental Microbiology. 2013 ; Vol. 15, No. 11. pp. 2937-2950.
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