The first intron of human H-ras is regulated by p53

Mediation of specific activation by a p53-binding element

W. Zhang, G. S. Randhawa, J. P. Gau, J. W. Shay, A. B. Deisseroth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We conducted transient reporter gene expression assays to show that the wild-type but not the mutant-type p53 protein positively regulates the expression of the human H-ras gene. This activation of expression is mediated through a specific p53-binding DNA element located in the first intron of the H-ras gene. This element is similar to a previously identified p53-binding element. Without this element, wild-type p53 represses the H-ras promoter, as do several p53 mutants. The repression function is lost when the N-terminal 81 amino acids of the p53 protein are deleted, suggesting that the N-terminus is required for repression. Therefore, p53 seems to regulate the H-ras through both positive and negative mechanisms.

Original languageEnglish (US)
Pages (from-to)1021-1028
Number of pages8
JournalInternational Journal of Oncology
Volume7
Issue number5
StatePublished - 1995

Fingerprint

ras Genes
Introns
Mutant Proteins
Reporter Genes
Gene Expression
Amino Acids
DNA
Proteins

Keywords

  • p53
  • Transcription
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The first intron of human H-ras is regulated by p53 : Mediation of specific activation by a p53-binding element. / Zhang, W.; Randhawa, G. S.; Gau, J. P.; Shay, J. W.; Deisseroth, A. B.

In: International Journal of Oncology, Vol. 7, No. 5, 1995, p. 1021-1028.

Research output: Contribution to journalArticle

Zhang, W. ; Randhawa, G. S. ; Gau, J. P. ; Shay, J. W. ; Deisseroth, A. B. / The first intron of human H-ras is regulated by p53 : Mediation of specific activation by a p53-binding element. In: International Journal of Oncology. 1995 ; Vol. 7, No. 5. pp. 1021-1028.
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