The G protein β₂ complementary DNA encodes the β₃₅ subunit

Antisera were generated against synthetic peptides that correspond to amino acid sequences deduced from a cDNA (designated β₂) that encodes a second form of the β subunit of guanine nucleotide-binding regulatory proteins (G proteins). The specificity of interactions of these antisera with purified G protein β subunits indicates that the β₂ cDNA encodes the β₃₅ form of this polypeptide. This hypothesis is confirmed by the use of these antisera to detect expression of the β₂ cDNA in COS-m6 cells.