The G protein β2 complementary DNA encodes the β35 subunit

B. Gao, S. Mumby, A. G. Gilman

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Antisera were generated against synthetic peptides that correspond to amino acid sequences deduced from a cDNA (designated β2) that encodes a second form of the β subunit of guanine nucleotide-binding regulatory proteins (G proteins). The specificity of interactions of these antisera with purified G protein β subunits indicates that the β2 cDNA encodes the β35 form of this polypeptide. This hypothesis is confirmed by the use of these antisera to detect expression of the β2 cDNA in COS-m6 cells.

Original languageEnglish (US)
Pages (from-to)17254-17257
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number36
StatePublished - 1987

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Guanine Nucleotides
GTP-Binding Proteins
Immune Sera
Carrier Proteins
Complementary DNA
Peptides
Proteins
COS Cells
Protein Subunits
Amino Acid Sequence
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

The G protein β2 complementary DNA encodes the β35 subunit. / Gao, B.; Mumby, S.; Gilman, A. G.

In: Journal of Biological Chemistry, Vol. 262, No. 36, 1987, p. 17254-17257.

Research output: Contribution to journalArticle

Gao, B, Mumby, S & Gilman, AG 1987, 'The G protein β2 complementary DNA encodes the β35 subunit', Journal of Biological Chemistry, vol. 262, no. 36, pp. 17254-17257.
Gao, B. ; Mumby, S. ; Gilman, A. G. / The G protein β2 complementary DNA encodes the β35 subunit. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 36. pp. 17254-17257.
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