The Gal4 Activation Domain Binds Sug2 Protein, a Proteasome Component, in Vivo and in Vitro

Cathy Chang, Fernando Gonzalez, Beverly Rothermel, Liping Sun, Stephen Albert Johnston, Thomas Kodadek

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

An in vivo protein interaction assay was used to search a yeast cDNA library for proteins that bind to the acidic activation domain (AD) of the yeast Gal4 protein. Sug2 protein, a component of the 19 S regulatory particle of the 26 S proteasome, was one of seven proteins identified in this screen. In vitro binding assays confirm a direct interaction between these proteins. SUG2 and SUG1, another 19 S component, were originally discovered as a mutation able to suppress the phenotype of a Gal4 truncation mutant (Gal4Dp) lacking much of its AD. Sug1p has previously been shown to bind the Gal4 AD in vitro. Taken together, these genetic and biochemical data suggest a biologically significant interaction between the Gal4 protein and the 19 S regulatory particle of the proteasome. Indeed, it is demonstrated here that the Gal4 AD interacts specifically with immunopurified 19 S complex. The proteasome regulatory particle has been shown recently to play a direct role in RNA polymerase II transcription and the activator-19 S interaction could be important in recruiting this large complex to transcriptionally active GAL genes.

Original languageEnglish (US)
Pages (from-to)30956-30963
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number33
DOIs
StatePublished - Aug 17 2001

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Proteasome Endopeptidase Complex
Chemical activation
Proteins
Assays
Fungal Proteins
RNA Polymerase II
Protein S
Gene Library
Transcription
Molecular Biology
Yeast
Yeasts
In Vitro Techniques
Phenotype
Genes
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Gal4 Activation Domain Binds Sug2 Protein, a Proteasome Component, in Vivo and in Vitro. / Chang, Cathy; Gonzalez, Fernando; Rothermel, Beverly; Sun, Liping; Johnston, Stephen Albert; Kodadek, Thomas.

In: Journal of Biological Chemistry, Vol. 276, No. 33, 17.08.2001, p. 30956-30963.

Research output: Contribution to journalArticle

Chang, Cathy ; Gonzalez, Fernando ; Rothermel, Beverly ; Sun, Liping ; Johnston, Stephen Albert ; Kodadek, Thomas. / The Gal4 Activation Domain Binds Sug2 Protein, a Proteasome Component, in Vivo and in Vitro. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 33. pp. 30956-30963.
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