The Golgi ‘casein kinase’ Fam20C is a genuine ‘phosvitin kinase’ and phosphorylates polyserine stretches devoid of the canonical consensus

Giorgio Cozza, Enrico Moro, Miles Black, Oriano Marin, Mauro Salvi, Andrea Venerando, Vincent S Tagliabracci, Lorenzo A. Pinna

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Egg yolk phosvitins, generated through the fragmentation of vitellogenins (VTGs), are among the most heavily phosphorylated proteins ever described. Despite the early discovery in 1900 that chicken phosvitin is a phosphoprotein and its subsequent employment as an artificial substrate for a number of protein kinases, the identity of the enzyme(s) responsible for its phosphorylation remained a matter of conjecture until present. Here, we provide evidence that phosvitin phosphorylation is catalyzed by a family with sequence similarity 20, member C (Fam20C), an atypical protein kinase recently identified as the genuine casein kinase and responsible for the phosphorylation of many other secreted proteins at residues specified by the S-x-E/pS consensus. Such a conclusion is grounded on the following observations: (a) the levels of Fam20C and phosphorylated VTG rise in parallel upon treatment of zebrafish with oestrogens; (b) zebrafish phosvitin is readily phosphorylated upon coexpression in U2OS cells with Fam20C, but not with its catalytically inactive mutant; (c) a peptide reproducing a stretch of 12 serines, which are phosphorylated in chicken phosvitin despite lacking the C-terminal priming motif S-x-E, is efficiently phosphorylated by both recombinant and native Fam20C. The last finding expands the repertoire of potential targets of Fam20C to include several proteins known to harbor (p-Ser)n clusters not specified by any known kinase consensus.

Original languageEnglish (US)
JournalFEBS Journal
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Phosvitin
Casein Kinases
Consensus
Phosphotransferases
Phosphorylation
Vitellogenins
Zebrafish
Chickens
Proteins
Egg Yolk
Phosphoproteins
Ports and harbors
Protein Kinases
Serine
Estrogens
polyserine
Peptides
Substrates
Enzymes

Keywords

  • Fam20C
  • phosphoserine stretches
  • phosvitin
  • sphingolipid signaling
  • vitellogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The Golgi ‘casein kinase’ Fam20C is a genuine ‘phosvitin kinase’ and phosphorylates polyserine stretches devoid of the canonical consensus. / Cozza, Giorgio; Moro, Enrico; Black, Miles; Marin, Oriano; Salvi, Mauro; Venerando, Andrea; Tagliabracci, Vincent S; Pinna, Lorenzo A.

In: FEBS Journal, 01.01.2018.

Research output: Contribution to journalArticle

Cozza, Giorgio ; Moro, Enrico ; Black, Miles ; Marin, Oriano ; Salvi, Mauro ; Venerando, Andrea ; Tagliabracci, Vincent S ; Pinna, Lorenzo A. / The Golgi ‘casein kinase’ Fam20C is a genuine ‘phosvitin kinase’ and phosphorylates polyserine stretches devoid of the canonical consensus. In: FEBS Journal. 2018.
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