The green tea polyphenol ()-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis

Ai Peng, Ting Ye, Dinesh Rakheja, Yangke Tu, Tao Wang, Yong Du, Jason K. Zhou, Nosratola D. Vaziri, Zhao Hu, Chandra Mohan, Xin J. Zhou

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The unchecked overproduction of reactive oxygen and nitrogen species by inflammatory cells can cause tissue damage, intensify inflammation, promote apoptosis, and accelerate the progression of immune-mediated glomerulonephritis (GN). Here we tested whether the anti-inflammatory and antioxidant properties of the green tea polyphenol ()-epigallocatechin-3-gallate (EGCG) favorably affect the development of immune-mediated GN. Pretreatment of 129/svJ mice with EGCG from 2 days before to 2 weeks after the induction of GN led to reduced proteinuria and serum creatinine, and marked improvement in renal histology when compared with vehicle-pretreated diseased mice. This pretreatment reduced oxidative stress, and normalized osteopontin, p65/nuclear factor-B, inducible nitric oxide synthase, nitric oxide metabolites, p-Akt, phosphorylated extracellular signal-regulated kinases 1 and 2, p47phox, and myeloperoxidase, all of which were elevated in vehicle-pretreated diseased mice. Levels of glutathione peroxidase and peroxisome proliferator-activated receptor-γ (PPARγ), both reduced in the vehicle-pretreated diseased mice, were normalized. This renoprotective effect was reversed by concomitant administration of the PPARγ antagonist GW9662 throughout the EGCG pretreatment period. Importantly, mortality and renal dysfunction were significantly attenuated even when the polyphenol treatment was initiated 1 week after the onset of GN. Thus, EGCG reversed the progression of immune-mediated GN in mice by targeting redox and inflammatory pathways.

Original languageEnglish (US)
Pages (from-to)601-611
Number of pages11
JournalKidney International
Volume80
Issue number6
DOIs
StatePublished - Sep 2 2011

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Polyphenols
Tea
Glomerulonephritis
Peroxisome Proliferator-Activated Receptors
129 Strain Mouse
Kidney
Reactive Nitrogen Species
Osteopontin
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Nitric Oxide Synthase Type II
Glutathione Peroxidase
Proteinuria
Peroxidase
Oxidation-Reduction
Reactive Oxygen Species
Creatinine
Histology
Nitric Oxide
Oxidative Stress

Keywords

  • anti-GBM disease
  • nitric oxide
  • oxidative stress
  • reactive oxygen species
  • renal pathology

ASJC Scopus subject areas

  • Nephrology

Cite this

The green tea polyphenol ()-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. / Peng, Ai; Ye, Ting; Rakheja, Dinesh; Tu, Yangke; Wang, Tao; Du, Yong; Zhou, Jason K.; Vaziri, Nosratola D.; Hu, Zhao; Mohan, Chandra; Zhou, Xin J.

In: Kidney International, Vol. 80, No. 6, 02.09.2011, p. 601-611.

Research output: Contribution to journalArticle

Peng, A, Ye, T, Rakheja, D, Tu, Y, Wang, T, Du, Y, Zhou, JK, Vaziri, ND, Hu, Z, Mohan, C & Zhou, XJ 2011, 'The green tea polyphenol ()-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis', Kidney International, vol. 80, no. 6, pp. 601-611. https://doi.org/10.1038/ki.2011.121
Peng, Ai ; Ye, Ting ; Rakheja, Dinesh ; Tu, Yangke ; Wang, Tao ; Du, Yong ; Zhou, Jason K. ; Vaziri, Nosratola D. ; Hu, Zhao ; Mohan, Chandra ; Zhou, Xin J. / The green tea polyphenol ()-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. In: Kidney International. 2011 ; Vol. 80, No. 6. pp. 601-611.
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