The head involution defective gene of Drosophila melanogaster functions in programmed cell death

M. E. Grether, J. M. Abrams, J. Agapite, K. White, H. Steller

Research output: Contribution to journalArticle

553 Citations (Scopus)

Abstract

Deletions of chromosomal region, 75C1,2 block virtually all programmed cell death (PCD) in the Drosophila embryo. We have identified a gene previously in this interval, reaper (rpr), which encodes an important regulator of PCD. Here we report the isolation of a second gene in this region, head involution defective (hid), which plays a similar role in PCD. hid mutant embryos have decreased levels of cell death and contain extra cells in the head. We have cloned the hid gene and find that its expression is sufficient to induce PCD in cell death defective mutants. The hid gene appears to encode a novel 410-amino-acid protein, and its mRNA is expressed in regions of the embryo where cell death occurs. Ectopic expression of hid in the Drosophila retina results in eye ablation. This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid.

Original languageEnglish (US)
Pages (from-to)1694-1708
Number of pages15
JournalGenes and Development
Volume9
Issue number14
StatePublished - 1995

Fingerprint

Drosophila melanogaster
Cell Death
Head
Genes
Drosophila
Embryonic Structures
Embryo Loss
Apoptosis Regulatory Proteins
Baculoviridae
Retina
Phenotype
Amino Acids
Messenger RNA
Proteins

Keywords

  • apoptosis
  • Drosophila
  • programmed cell death

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

The head involution defective gene of Drosophila melanogaster functions in programmed cell death. / Grether, M. E.; Abrams, J. M.; Agapite, J.; White, K.; Steller, H.

In: Genes and Development, Vol. 9, No. 14, 1995, p. 1694-1708.

Research output: Contribution to journalArticle

Grether, ME, Abrams, JM, Agapite, J, White, K & Steller, H 1995, 'The head involution defective gene of Drosophila melanogaster functions in programmed cell death', Genes and Development, vol. 9, no. 14, pp. 1694-1708.
Grether, M. E. ; Abrams, J. M. ; Agapite, J. ; White, K. ; Steller, H. / The head involution defective gene of Drosophila melanogaster functions in programmed cell death. In: Genes and Development. 1995 ; Vol. 9, No. 14. pp. 1694-1708.
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