The hemispheric functional expression of the thyrotropin-releasing-hormone receptor is not determined by the receptors' physical distribution

N. Matus-Leibovitch, D. R. Nussenzveig, M. C. Gershengorn, Y. Oron

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Abstract

The thyrotropin-releasing-hormone receptor (TRH-R) is a member of a family of the G-protein-coupled receptors that share structural similarities and exert their physiological action via the inositol lipid signal-transduction pathway. The TRH-R when expressed in Xenopus oocytes exhibits marked preference of the response (increased chloride conductance) for the animal hemisphere. Whereas the rat TRH-R functional distribution was strongly asymmetric (animal/vegetal ratio = 9.5), the mouse TRH-R exhibited a significantly lower ratio (3.9). Truncation of the last 59 amino acids of the C-terminal region of the mouse TRH-R did not lead to any changes in the functional hemispheric distribution. Despite the polarization of response, receptor number was similar on both hemispheres. Moreover, the apparent half-life of the functional expression of the TRH-R was approx. 4 h on both hemispheres when the expression was inhibited by a specific antisense oligonucleotide. Inhibition of total protein synthesis with cycloheximide affected hemispheric responses mediated by each of the three TRH-Rs tested in a qualitatively different way. These results suggest that an additional, rapidly degraded, protein modulates the functional hemispheric expression of the TRH-Rs.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalBiochemical Journal
Volume303
Issue number1
StatePublished - 1994

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Thyrotropin Releasing Hormone Receptors
Animals
Signal transduction
Antisense Oligonucleotides
Inositol
Cycloheximide
G-Protein-Coupled Receptors
Xenopus
Oocytes
Half-Life
Rats
Chlorides
Signal Transduction
Proteins
Polarization
Lipids
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

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The hemispheric functional expression of the thyrotropin-releasing-hormone receptor is not determined by the receptors' physical distribution. / Matus-Leibovitch, N.; Nussenzveig, D. R.; Gershengorn, M. C.; Oron, Y.

In: Biochemical Journal, Vol. 303, No. 1, 1994, p. 129-134.

Research output: Contribution to journalArticle

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