The HL-60 transforming sequence: A ras oncogene coexisting with altered myc genes in hematopoietic tumors

Mark J. Murray, James M. Cunningham, Luis F. Parada, Francois Dautry, Paul Lebowitz, Robert A. Weinberg

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Abstract

The oncogene of the HL-60 human promyelocytic leukemia cell line has been passed serially through NIH/3T3 mouse fibroblasts. Oncogene-specific probes prepared from the resulting tertiary transfectants by molecular cloning have been used to show that loss of the transfected oncogene from NIH/3T3 cells correlates with reversion to nontransformed morphology. Analysis of cells transfected by the oncogenes of other tumors and tumor cell lines indicates that the transforming gene of the HL-60 leukemia cell line is closely related to oncogenes of a Burkitt's lymphoma, an acute myelogenous leukemia, an adenocarcinoma of the colon, a neuroblastoma, and two sarcomas. This oncogene is distantly related to the viral oncogenes of Kirsten and Harvey sarcoma viruses. It has been termed N-ras. The active N-ras oncogene coexists with altered versions of the myc oncogene in the HL-60 and AW Ramos human tumors. This suggests a multistep mechanism involving both ras and myc genes in the creation of these tumors.

Original languageEnglish (US)
Pages (from-to)749-757
Number of pages9
JournalCell
Volume33
Issue number3
DOIs
Publication statusPublished - 1983

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Murray, M. J., Cunningham, J. M., Parada, L. F., Dautry, F., Lebowitz, P., & Weinberg, R. A. (1983). The HL-60 transforming sequence: A ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell, 33(3), 749-757. https://doi.org/10.1016/0092-8674(83)90017-X