The HLA-dependent expression of testis-organizing H-Y antigen by human male cells

Bruce Beutler, Yukifumi Nagai, Susumu Ohno, George Klein, Ilya M. Shapiro

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The proposal that the stable expression of organogenesis-directing plasma membrane antigens, such as testis-organizing H-Y antigen, requires β2-microglobulin-MHC antigen dimers as anchorage sites was tested on Daudi human Burkitt lymphoma cells [46, XY, 15q-, 14q+, β2-m(-), HLA(-)]. The H-Y antigen level of Daudi was only 20% of that of Raji and Ramos, two human male pseudodiploid Burkitt lymphoma lines that were β2-m(+), HLA(+). When Daudi is hybridized with β2-m(+), HLA(+) cell lines, β2-microglobulin, supplied by the latter, is known to restore the expression of Daudi HLA antigens A10 and BW17. Such restoration of HLA antigen expression markedly elevated H-Y antigen levels in those somatic hybrids. Thus the H-Y antigen level of the Daudi × Raji 8A (♂ × ♂) hybrid became equal to that of TetraRaji-the colcemide-induced Raji tetraploid line. Two independently derived Daudi × HeLa D98 (♂ × ♀) hybrids, DAD 1 and DAD 10, demonstrated even higher H-Y antigen levels comparable to that of normal male peripheral blood lymphocytes.

Original languageEnglish (US)
Pages (from-to)509-513
Number of pages5
JournalCell
Volume13
Issue number3
DOIs
StatePublished - 1978

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H-Y Antigen
Testis
Burkitt Lymphoma
Demecolcine
Antigens
Tetraploidy
Organogenesis
Lymphocytes
Cell membranes
HLA Antigens
Dimers
Restoration
Blood
Cells
Cell Membrane
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The HLA-dependent expression of testis-organizing H-Y antigen by human male cells. / Beutler, Bruce; Nagai, Yukifumi; Ohno, Susumu; Klein, George; Shapiro, Ilya M.

In: Cell, Vol. 13, No. 3, 1978, p. 509-513.

Research output: Contribution to journalArticle

Beutler, Bruce ; Nagai, Yukifumi ; Ohno, Susumu ; Klein, George ; Shapiro, Ilya M. / The HLA-dependent expression of testis-organizing H-Y antigen by human male cells. In: Cell. 1978 ; Vol. 13, No. 3. pp. 509-513.
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