The hMSH2(M688R) lynch syndrome mutation may function as a dominant negative

Juana V. Martín-López, Ysamar Barrios, Vicente Medina-arana, Miguel Andújar, Sanghee Lee, Liya Gu, Guo Min Li, Josef Rüschoff, Eduardo Salido, Richard Fishel

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The hMSH2(M688R) mismatch repair (MMR) gene mutation has been found in five large families from Tenerife, Spain, suggesting it is a Lynch syndrome or hereditary non-polyposis colorectal cancer (LS/HNPCC) founder mutation. In addition to classical LS/HNPCC tumors, these families present with a high incidence of central nervous system (CNS) tumors normally associated with Turcot or constitutional mismatch repair deficiency (CMMR-D) syndromes. Turcot and CMMR-D mutations may be biallelic, knocking out both copies of the MMR gene. The hMSH2(M688R) mutation is located in the ATP hydrolysis (ATPase) domain. We show that the hMSH2(M688R)-hMSH6 heterodimer binds to mismatched nucleotides but lacks normal ATP functions and inhibits MMR in vitro when mixed with the wild-type (WT) heterodimer. Another alteration that has been associated with LS/HNPCC, hMSH2(M688I)-hMSH6, displays no identifiable differences with the WT heterodimer. Interestingly, some extracolonic tumors from hMSH2(M688R) carriers may express hMSH2-hMSH6, yet display microsatellite instability (MSI). The functional analysis along with variability in tumor expression and the high incidence of CNS tumors suggests that hMSH2(M688R) may act as a dominant negative in some tissues, while the hMSH2(M688I) is most likely a benign polymorphism.

Original languageEnglish (US)
Pages (from-to)1647-1654
Number of pages8
JournalCarcinogenesis
Volume33
Issue number9
DOIs
StatePublished - Sep 2012

ASJC Scopus subject areas

  • Cancer Research

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