The human and mouse homologs of the yeast RAD52 gene

cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues

Zhiyuan Shen, Karen Denison, Rebecca Lobb, Joe M. Gatewood, David J. Chen

Research output: Contribution to journalArticle

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Abstract

The yeast Saccharomyces cerevisiae RAD52 gene is involved in DNA double-strand break repair and mitotic/meiotic recombination. The N-terminal amino acid sequence of yeast S. cerevisiae, Schizosaccharomyces pombe, and Kluyveromyces lactis and chicken is highly conserved. Using the technology of mixed oligonucleotide primed amplification of cDNA (MOPAC), two mouse RAD52 homologous cDNA fragments were amplified and sequenced. Subsequently, we have cloned the cDNA of the human and mouse homologs of yeast RAD52 gene by screening cDNA libraries using the identified mouse cDNA fragments. Sequence analysis of cDNA derived amino acid revealed a highly conserved N-terminus among human, mouse, chicken, and yeast RAD52 genes. The human RAD52 gene was assigned to chromosome 12p12.2-p13 by fluorescence in situ hybridization, R-banding, and DNA analysis of somatic cell hybrids. Unlike chicken RAD52 and mouse RAD51, no significant difference in mouse RAD52 mRNA level was found among mouse heart, brain, spleen, lung, liver, skeletal muscle, kidney, and testis. In addition to an ∼1.9-kb RAD52 mRNA band that is present in all of the tested tissues, an extra mRNA species of ∼0.85 kb was detectable in mouse testis.

Original languageEnglish (US)
Pages (from-to)199-206
Number of pages8
JournalGenomics
Volume25
Issue number1
DOIs
StatePublished - Jan 1 1995

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Human Chromosomes
Sequence Analysis
Organism Cloning
Complementary DNA
Yeasts
Messenger RNA
Genes
Chickens
Saccharomyces cerevisiae
Testis
Kluyveromyces
Double-Stranded DNA Breaks
Schizosaccharomyces
Hybrid Cells
Fluorescence In Situ Hybridization
Gene Library
Oligonucleotides
Genetic Recombination
Amino Acid Sequence
Skeletal Muscle

ASJC Scopus subject areas

  • Genetics

Cite this

The human and mouse homologs of the yeast RAD52 gene : cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues. / Shen, Zhiyuan; Denison, Karen; Lobb, Rebecca; Gatewood, Joe M.; Chen, David J.

In: Genomics, Vol. 25, No. 1, 01.01.1995, p. 199-206.

Research output: Contribution to journalArticle

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