The human umbilical vein as a biologic scaffold for vocal fold reconstruction

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

A variety of extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines the potential of the HUV as an acellular scaffold for engineering the vocal fold, in order to develop an implantable tissue substitute that can promote constructive tissue remodeling. A saline-based decellularization protocol was used to fabricate a biodegradable, acellular, three-dimensional scaffold from native HUV tissue. Histological examination and scanning electron microscopy demonstrated that native cellular materials in the HUV were removed with a fine three-dimensional structure of proteins and proteoglycans well preserved. Primary human vocal fold fibroblasts were seeded on the abluminal surface of the acellular HUV scaffold and cultured for 21 days. Significant proliferation and infiltration of the fibroblasts in the scaffold were observed. These data supported the promise of the HUV scaffold for vocal fold regeneration and restoration.

Original languageEnglish (US)
Title of host publicationProceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
DOIs
StatePublished - Jun 8 2010
Event36th Annual Northeast Bioengineering Conference, NEBEC 2010 - New York, NY, United States
Duration: Mar 26 2010Mar 28 2010

Publication series

NameProceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010

Other

Other36th Annual Northeast Bioengineering Conference, NEBEC 2010
CountryUnited States
CityNew York, NY
Period3/26/103/28/10

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ASJC Scopus subject areas

  • Bioengineering

Cite this

Chan, R. W. (2010). The human umbilical vein as a biologic scaffold for vocal fold reconstruction. In Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010 [5458281] (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010). https://doi.org/10.1109/NEBC.2010.5458281