The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length

Emily L. Hunter; Karl Lechtreck; Gang Fu; Juyeon Hwang; Huawen Lin; Avanti Gokhale; Lea M. Alford; Brian Lewis; Ryosuke Yamamoto; Ritsu Kamiya; Fan Yang; Daniela Nicastro; Susan K. Dutcher; Maureen Wirschell; Winfield S. Sale; Erika Holzbaur

doi:10.1091/mbc.E17-12-0729

The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length

Emily L. Hunter, Karl Lechtreck, Gang Fu, Juyeon Hwang, Huawen Lin, Avanti Gokhale, Lea M. Alford, Brian Lewis, Ryosuke Yamamoto, Ritsu Kamiya, Fan Yang, Daniela Nicastro, Susan K. Dutcher, Maureen Wirschell, Winfield S. Sale, Erika Holzbaur

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Abstract

Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.

Original language	English (US)
Pages (from-to)	886-896
Number of pages	11
Journal	Molecular biology of the cell
Volume	29
Issue number	8
DOIs	https://doi.org/10.1091/mbc.E17-12-0729
State	Published - Apr 15 2018

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1091/mbc.E17-12-0729

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Hunter, E. L., Lechtreck, K., Fu, G., Hwang, J., Lin, H., Gokhale, A., Alford, L. M., Lewis, B., Yamamoto, R., Kamiya, R., Yang, F., Nicastro, D., Dutcher, S. K., Wirschell, M., Sale, W. S., & Holzbaur, E. (2018). The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length. Molecular biology of the cell, 29(8), 886-896. https://doi.org/10.1091/mbc.E17-12-0729

Hunter, EL, Lechtreck, K, Fu, G, Hwang, J, Lin, H, Gokhale, A, Alford, LM, Lewis, B, Yamamoto, R, Kamiya, R, Yang, F, Nicastro, D, Dutcher, SK, Wirschell, M, Sale, WS & Holzbaur, E 2018, 'The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length', Molecular biology of the cell, vol. 29, no. 8, pp. 886-896. https://doi.org/10.1091/mbc.E17-12-0729

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abstract = "Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.",

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AB - Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.

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The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length

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