@article{5b7450b114e94f87979917d8c7df92f1,
title = "The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length",
abstract = "Axonemal dyneins, including inner dynein arm I1, assemble in the cytoplasm prior to transport into cilia by intraflagellar transport (IFT). How I1 dynein interacts with IFT is not understood. We take advantage of the Chlamydomonas reinhardtii ida3 mutant, which assembles the inner arm I1 dynein complex in the cytoplasm but fails to transport I1 into the cilium, resulting in I1 dynein-deficient axonemes with abnormal motility. The IDA3 gene encodes an ∼115-kDa coiled-coil protein that primarily enters the cilium during ciliary growth but is not an axonemal protein. During growth, IDA3, along with I1 dynein, is transported by anterograde IFT to the tip of the cilium. At the tip, IDA3 uncouples from IFT and diffuses within the cilium. IFT transport of IDA3 decreases as cilia lengthen and subsides once full length is achieved. IDA3 is the first example of an essential and selective IFT adapter that is regulated by ciliary length.",
author = "Hunter, {Emily L.} and Karl Lechtreck and Gang Fu and Juyeon Hwang and Huawen Lin and Avanti Gokhale and Alford, {Lea M.} and Brian Lewis and Ryosuke Yamamoto and Ritsu Kamiya and Fan Yang and Daniela Nicastro and Dutcher, {Susan K.} and Maureen Wirschell and Sale, {Winfield S.} and Erika Holzbaur",
note = "Funding Information: This research was supported by funding from the National Institutes of Health (NIH) (W.S.S. and M.W., GM051173; K.L., GM1100413; D.N., GM083122; and S.K.D., HL128370) and the Children's Discovery Institute, PD-II-2014-379 to S.K.D. E.L.H. was supported by a Greater Southeast Affiliate Pre-doctoral Fellowship from the American Heart Association (10PRE353007) and by a Biochemistry, Cell, and Molecular Biology (BCMB) Training Grant at Emory University (NIH T32 GM008367). A.G. was supported in part by an Emory University Catalyst Grant. This study was also supported in part by the Emory Integrated Genomics Core (EIGC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities. R.Y. was the recipient of a Postdoctoral Fellowship for Research Abroad from the Japan Society for the Promotion of Science (JSPS). Additional support for sequencing was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award No. UL1TR000454. We are grateful to T. Oda (Department of Anatomy and Structural Biology, Graduate School of Medical Science, University of Yamanashi) for the gift of the ida7; IC140::GFP cells. The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institutes of Health. Funding Information: This research was supported by funding from the National Institutes of Health (NIH) (W.S.S. and M.W., GM051173; K.L., GM1100413; D.N., GM083122; and S.K.D., HL128370) and the Children{\textquoteright}s Discovery Institute, PD-II-2014-379 to S.K.D. E.L.H. was supported by a Greater Southeast Affiliate Pre-doctoral Fellowship from the American Heart Association (10PRE353007) and by a Biochemistry, Cell, and Molecular Biology (BCMB) Training Grant at Emory University (NIH T32 GM008367). A.G. was supported in part by an Emory University Catalyst Grant. This study was also supported in part by the Emory Integrated Genomics Core (EIGC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities. R.Y. was the recipient of a Postdoctoral Fellowship for Research Abroad from the Japan Society for the Promotion of Science (JSPS). Additional support for sequencing was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award No. UL1TR000454. We are grateful to T. Oda (Department of Anatomy and Structural Biology, Graduate School of Medical Science, University of Yamanashi) for the gift of the ida7; IC140::GFP cells. The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institutes of Health.",
year = "2018",
month = apr,
day = "15",
doi = "10.1091/mbc.E17-12-0729",
language = "English (US)",
volume = "29",
pages = "886--896",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "8",
}