The Ihog Cell-Surface Proteins Bind Hedgehog and Mediate Pathway Activation

Shenqin Yao, Lawrence Lum, Philip Beachy

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

Original languageEnglish (US)
Pages (from-to)343-357
Number of pages15
JournalCell
Volume125
Issue number2
DOIs
StatePublished - Apr 21 2006

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Hedgehog Proteins
Hedgehogs
Fibronectins
Membrane Proteins
Chemical activation
Drosophila
Embryo Loss
Imaginal Discs
RNA Interference
Genes
Cells
RNA
Ligands
Cultured Cells
Carrier Proteins
Defects
Mutation
Proteins
Fibronectin Type III Domain

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The Ihog Cell-Surface Proteins Bind Hedgehog and Mediate Pathway Activation. / Yao, Shenqin; Lum, Lawrence; Beachy, Philip.

In: Cell, Vol. 125, No. 2, 21.04.2006, p. 343-357.

Research output: Contribution to journalArticle

Yao, Shenqin ; Lum, Lawrence ; Beachy, Philip. / The Ihog Cell-Surface Proteins Bind Hedgehog and Mediate Pathway Activation. In: Cell. 2006 ; Vol. 125, No. 2. pp. 343-357.
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