The impact of the condenser on cytogenetic image quality in digital microscope system

Liqiang Ren, Zheng Li, Yuhua Li, Bin Zheng, Shibo Li, Xiaodong Chen, Hong Liu

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

BACKGROUND: Optimizing operational parameters of the digital microscope system is an important technique to acquire high quality cytogenetic images and facilitate the process of karyotyping so that the efficiency and accuracy of diagnosis can be improved. OBJECTIVE: This study investigated the impact of the condenser on cytogenetic image quality and system working performance using a prototype digital microscope image scanning system. METHODS: Both theoretical analysis and experimental validations through objectively evaluating a resolution test chart and subjectively observing large numbers of specimen were conducted. RESULTS: The results show that the optimal image quality and large depth of field (DOF) are simultaneously obtained when the numerical aperture of condenser is set as 60%-70% of the corresponding objective. Under this condition, more analyzable chromosomes and diagnostic information are obtained. As a result, the system shows higher working stability and less restriction for the implementation of algorithms such as autofocusing especially when the system is designed to achieve high throughput continuous image scanning. CONCLUSIONS: Although the above quantitative results were obtained using a specific prototype system under the experimental conditions reported in this paper, the presented evaluation methodologies can provide valuable guidelines for optimizing operational parameters in cytogenetic imaging using the high throughput continuous scanning microscopes in clinical practice.

Original languageEnglish (US)
Pages (from-to)45-59
Number of pages15
JournalAnalytical Cellular Pathology
Volume36
Issue number1-2
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • Optimizing operational parameters
  • cytogenetic imaging
  • depth of field (DOF)
  • image sharpness
  • microscope condenser
  • resolution

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine
  • Cell Biology
  • Cancer Research

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