The importance of cellular internalization of antibody-targeted carbon nanotubes in the photothermal ablation of breast cancer cells

Radu Marches, Carole Mikoryak, Ru Hung Wang, Paul Pantano, Rockford K. Draper, Ellen S. Vitetta

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Single-walled carbon nanotubes (CNTs) convert absorbed near infrared (NIR) light into heat. The use of CNTs in the NIR-mediated photothermal ablation of tumor cells is attractive because the penetration of NIR light through normal tissues is optimal and the side effects are minimal. Targeted thermal ablation with minimal collateral damage can be achieved by using CNTs attached to tumor-specific monoclonal antibodies (MAbs). However, the role that the cellular internalization of CNTs plays in the subsequent sensitivity of the target cells to NIR-mediated photothermal ablation remains undefined. To address this issue, we used CNTs covalently coupled to an anti-Her2 or a control MAb and tested their ability to bind, internalize, and photothermally ablate Her2+ but not Her2- breast cancer cell lines. Using flow cytometry, immunofluorescence, and confocal Raman microscopy, we observed the gradual time-dependent receptor-mediated endocytosis of anti-Her2-CNTs whereas a control MAb-CNT conjugate did not bind to the cells. Most importantly, the Her2 + cells that internalized the MAb-CNTs were more sensitive to NIR-mediated photothermal damage than cells that could bind to, but not internalize the MAb-CNTs. These results suggest that both the targeting and internalization of MAb-CNTs might result in the most effective thermal ablation of tumor cells following their exposure to NIR light.

Original languageEnglish (US)
Article number095101
JournalNanotechnology
Volume22
Issue number9
DOIs
StatePublished - Mar 4 2011

Fingerprint

Carbon Nanotubes
Ablation
Antibodies
Carbon nanotubes
Cells
Infrared radiation
Tumors
Monoclonal antibodies
Flow cytometry
Single-walled carbon nanotubes (SWCN)
Microscopic examination
Monoclonal Antibodies
Tissue

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

The importance of cellular internalization of antibody-targeted carbon nanotubes in the photothermal ablation of breast cancer cells. / Marches, Radu; Mikoryak, Carole; Wang, Ru Hung; Pantano, Paul; Draper, Rockford K.; Vitetta, Ellen S.

In: Nanotechnology, Vol. 22, No. 9, 095101, 04.03.2011.

Research output: Contribution to journalArticle

Marches, Radu ; Mikoryak, Carole ; Wang, Ru Hung ; Pantano, Paul ; Draper, Rockford K. ; Vitetta, Ellen S. / The importance of cellular internalization of antibody-targeted carbon nanotubes in the photothermal ablation of breast cancer cells. In: Nanotechnology. 2011 ; Vol. 22, No. 9.
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