The interaction of myristylated peptides with the catalytic domain of protein kinase C revealed by their sequence palindromy and the identification of a myristyl binding site

Andrea Zaliani, M. Pinori, H. L. Ball, G. DiGregorio, P. Cremonesi, Paolo Mascagni

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using a model of the enzyme structure and the results from a series of free and myristylated peptides, we provide evidence that peptides corresponding to the pseudosubstrate sequence of protein kinase C bind to the enzyme substrate binding site in an essentially extended conformation. This and the nearly symmetrical location of positive charges around the substrate phosphoritable site allow the peptide to bind to the enzyme in either an N-to-C orientation or its C-to-N opposite orientation. The latter is favoured by a change in residue chirality or when the peptide bears a myristoyl chain at its N-terminus. A myristyl binding site was also identified in the enzyme structure and its location in a region proximal to the C-terminal residue of pseudosubstrate bound in the N-to-C direction suggested that C-myristylation of peptide substrates should be more effective than N-myristoylation in antagonizing the enzyme. A peptide (H-RFARKGALRQKN-CONH-Myr) which contains the myristyl chain covalently linked to the C-terminal residue of the pseudosubstrate was thus made and shown to be a potent inhibitor of the histone kinase reaction of protein kinase C and the phosphorylation of p47 in intact cells.

Original languageEnglish (US)
Pages (from-to)803-810
Number of pages8
JournalProtein Engineering
Volume11
Issue number9
StatePublished - Sep 1998

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Binding sites
Protein Kinase C
Peptides
Catalytic Domain
Identification (control systems)
Binding Sites
Proteins
Enzymes
Substrates
Protamine Kinase
Phosphorylation
Chirality
Conformations

Keywords

  • Myristyl binding site
  • Myristylated peptides
  • Peptide inhibitors
  • Protein kinase C

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

The interaction of myristylated peptides with the catalytic domain of protein kinase C revealed by their sequence palindromy and the identification of a myristyl binding site. / Zaliani, Andrea; Pinori, M.; Ball, H. L.; DiGregorio, G.; Cremonesi, P.; Mascagni, Paolo.

In: Protein Engineering, Vol. 11, No. 9, 09.1998, p. 803-810.

Research output: Contribution to journalArticle

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AU - Cremonesi, P.

AU - Mascagni, Paolo

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