The interactions of gallium with various buffers and chelating agents in aqueous solution: Gallium-71 and hydrogen-1 NMR studies

C. H Francis Chang, T. Phil Pitner, Robert E. Lenkinski, Jerry D. Glickson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The interactions of gallium (Ga) with the ligands, EDTA, NTA, phosphate, lactate, MOPS, TRIS and HEPES are investigated using both 71Ga and 1H nmr measurements. Both EDTA and NTA form strong complexes with gallium, which have a 1:1 stoichiometry. In alkaline solution the tetrahedral Ga(OD)4 - competes strongly with EDTA in complex formation. In the lactate complex, there are probably three lactates per gallium present. The phosphate complexes of gallium are difficult to characterize on the basis of this investigation. The buffers, MOPS, TRIS, and HEPES, do not interact with gallium significantly. The ability of the ligands to bind gallium correlates well with their ability to inhibit gallium incorporation by L1210 leukemic cells.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalBioinorganic Chemistry
Volume8
Issue number1
DOIs
StatePublished - 1978

Fingerprint

Gallium
Chelating Agents
Hydrogen
Buffers
Nuclear magnetic resonance
Edetic Acid
HEPES
Lactic Acid
Lactates
Phosphates
Ligands
Stoichiometry

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

The interactions of gallium with various buffers and chelating agents in aqueous solution : Gallium-71 and hydrogen-1 NMR studies. / Chang, C. H Francis; Pitner, T. Phil; Lenkinski, Robert E.; Glickson, Jerry D.

In: Bioinorganic Chemistry, Vol. 8, No. 1, 1978, p. 11-19.

Research output: Contribution to journalArticle

Chang, C. H Francis ; Pitner, T. Phil ; Lenkinski, Robert E. ; Glickson, Jerry D. / The interactions of gallium with various buffers and chelating agents in aqueous solution : Gallium-71 and hydrogen-1 NMR studies. In: Bioinorganic Chemistry. 1978 ; Vol. 8, No. 1. pp. 11-19.
@article{5acb518558894398a66580a0fb06bcac,
title = "The interactions of gallium with various buffers and chelating agents in aqueous solution: Gallium-71 and hydrogen-1 NMR studies",
abstract = "The interactions of gallium (Ga) with the ligands, EDTA, NTA, phosphate, lactate, MOPS, TRIS and HEPES are investigated using both 71Ga and 1H nmr measurements. Both EDTA and NTA form strong complexes with gallium, which have a 1:1 stoichiometry. In alkaline solution the tetrahedral Ga(OD)4 - competes strongly with EDTA in complex formation. In the lactate complex, there are probably three lactates per gallium present. The phosphate complexes of gallium are difficult to characterize on the basis of this investigation. The buffers, MOPS, TRIS, and HEPES, do not interact with gallium significantly. The ability of the ligands to bind gallium correlates well with their ability to inhibit gallium incorporation by L1210 leukemic cells.",
author = "Chang, {C. H Francis} and Pitner, {T. Phil} and Lenkinski, {Robert E.} and Glickson, {Jerry D.}",
year = "1978",
doi = "10.1016/S0006-3061(00)80001-0",
language = "English (US)",
volume = "8",
pages = "11--19",
journal = "Journal of Inorganic Biochemistry",
issn = "0162-0134",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - The interactions of gallium with various buffers and chelating agents in aqueous solution

T2 - Gallium-71 and hydrogen-1 NMR studies

AU - Chang, C. H Francis

AU - Pitner, T. Phil

AU - Lenkinski, Robert E.

AU - Glickson, Jerry D.

PY - 1978

Y1 - 1978

N2 - The interactions of gallium (Ga) with the ligands, EDTA, NTA, phosphate, lactate, MOPS, TRIS and HEPES are investigated using both 71Ga and 1H nmr measurements. Both EDTA and NTA form strong complexes with gallium, which have a 1:1 stoichiometry. In alkaline solution the tetrahedral Ga(OD)4 - competes strongly with EDTA in complex formation. In the lactate complex, there are probably three lactates per gallium present. The phosphate complexes of gallium are difficult to characterize on the basis of this investigation. The buffers, MOPS, TRIS, and HEPES, do not interact with gallium significantly. The ability of the ligands to bind gallium correlates well with their ability to inhibit gallium incorporation by L1210 leukemic cells.

AB - The interactions of gallium (Ga) with the ligands, EDTA, NTA, phosphate, lactate, MOPS, TRIS and HEPES are investigated using both 71Ga and 1H nmr measurements. Both EDTA and NTA form strong complexes with gallium, which have a 1:1 stoichiometry. In alkaline solution the tetrahedral Ga(OD)4 - competes strongly with EDTA in complex formation. In the lactate complex, there are probably three lactates per gallium present. The phosphate complexes of gallium are difficult to characterize on the basis of this investigation. The buffers, MOPS, TRIS, and HEPES, do not interact with gallium significantly. The ability of the ligands to bind gallium correlates well with their ability to inhibit gallium incorporation by L1210 leukemic cells.

UR - http://www.scopus.com/inward/record.url?scp=0017838444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017838444&partnerID=8YFLogxK

U2 - 10.1016/S0006-3061(00)80001-0

DO - 10.1016/S0006-3061(00)80001-0

M3 - Article

C2 - 23867

AN - SCOPUS:0017838444

VL - 8

SP - 11

EP - 19

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

SN - 0162-0134

IS - 1

ER -