The interactions of gallium with various buffers and chelating agents in aqueous solution: Gallium-71 and hydrogen-1 NMR studies

C. H Francis Chang, T. Phil Pitner, Robert E. Lenkinski, Jerry D. Glickson

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Abstract

The interactions of gallium (Ga) with the ligands, EDTA, NTA, phosphate, lactate, MOPS, TRIS and HEPES are investigated using both 71Ga and 1H nmr measurements. Both EDTA and NTA form strong complexes with gallium, which have a 1:1 stoichiometry. In alkaline solution the tetrahedral Ga(OD)4 - competes strongly with EDTA in complex formation. In the lactate complex, there are probably three lactates per gallium present. The phosphate complexes of gallium are difficult to characterize on the basis of this investigation. The buffers, MOPS, TRIS, and HEPES, do not interact with gallium significantly. The ability of the ligands to bind gallium correlates well with their ability to inhibit gallium incorporation by L1210 leukemic cells.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalBioinorganic Chemistry
Volume8
Issue number1
DOIs
Publication statusPublished - 1978

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ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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