The interplay of cell-cell and cell-substrate adhesion in collective cell migration

Chenlu Wang, Sagar Chowdhury, Meghan Driscoll, Carole A. Parent, S. K. Gupta, Wolfgang Losert

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Collective cell migration often involves notable cell-cell and cell-substrate adhesions and highly coordinated motion of touching cells. We focus on the interplay between cell-substrate adhesion and cell-cell adhesion. We show that the loss of cell-surface contact does not significantly alter the dynamic pattern of protrusions and retractions of fast migrating amoeboid cells (Dictyostelium discoideum), but significantly changes their ability to adhere to other cells. Analysis of the dynamics of cell shapes reveals that cells that are adherent to a surface may coordinate their motion with neighbouring cells through protrusion waves that travel across cell-cell contacts. However, while shape waves exist if cells are detached from surfaces, they do not couple cell to cell. In addition, our investigation of actin polymerization indicates that loss of cell-surface adhesion changes actin polymerization at cell-cell contacts. To further investigate cell-cell/cell-substrate interactions, we used optical micromanipulation to form cell-substrate contact at controlled locations. We find that both cell-shape dynamics and cytoskeletal activity respond rapidly to the formation of cell-substrate contact.

Original languageEnglish (US)
Article number0684
JournalJournal of the Royal Society Interface
Volume11
Issue number100
DOIs
StatePublished - Nov 6 2014
Externally publishedYes

Fingerprint

Cell Adhesion
Cell Movement
Adhesion
Substrates
Actins
Polymerization
Cell adhesion
Cells
Cell Shape
Micromanipulation
Dictyostelium

Keywords

  • Actin polymerization
  • Cell migration
  • Cell-cell adhesion
  • Cell-shape dynamics
  • Cell-substrate adhesion
  • Dictyostelium discoideum

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

Cite this

The interplay of cell-cell and cell-substrate adhesion in collective cell migration. / Wang, Chenlu; Chowdhury, Sagar; Driscoll, Meghan; Parent, Carole A.; Gupta, S. K.; Losert, Wolfgang.

In: Journal of the Royal Society Interface, Vol. 11, No. 100, 0684, 06.11.2014.

Research output: Contribution to journalArticle

Wang, Chenlu ; Chowdhury, Sagar ; Driscoll, Meghan ; Parent, Carole A. ; Gupta, S. K. ; Losert, Wolfgang. / The interplay of cell-cell and cell-substrate adhesion in collective cell migration. In: Journal of the Royal Society Interface. 2014 ; Vol. 11, No. 100.
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