The kinetics of V-J joining throughout 3.5 megabases of the mouse Igκ locus fit a constrained diffusion model of nuclear organization

Shuyu Li, William T. Garrard

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To gain insight into the nuclear organization of the mouse Igκ locus and how it may relate to the formation of synapses during recombination, we have studied the kinetics of rearrangement of different Vκ gene families to Jκ gene segments in the pre-B cell line, 103bcl2. Remarkably, Vκ gene families separated by more than 3.5 Mb from Jκ gene segments rearranged with nearly identical kinetics to those as close as 18 kb to Jκ gene segments. These results fit a model of nuclear organization in which the entire VκJκ region resides within a single nuclear subcompartment and is capable of exhibiting multiple reversible contacts through diffusion and Brownian motion.

Original languageEnglish (US)
Pages (from-to)125-129
Number of pages5
JournalFEBS Letters
Volume536
Issue number1-3
DOIs
StatePublished - Feb 11 2003

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Keywords

  • Igκ
  • Nuclear organization
  • Pre-B cell
  • RAG
  • Recombination

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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