The LDL receptor clustering motif interacts with the clathrin terminal domain in a reverse turn conformation

Richard G. Kibbey, Jose Rizo-Rey, Lila M. Gierasch, Richard G W Anderson

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Previously the hexapeptide motif FXNPXY807 in the cytoplasmic tail of the LDL receptor was shown to be essential for clustering in clathrin-coated pits. We used nuclear magnetic resonance line-broadening and transferred nuclear Overhauser effect measurements to identify the molecule in the clathrin lattice that interacts with this hexapeptide, and determined the structure of the bound motif. The wild-type peptide bound in a single conformation with a reverse turn at residues NPVY. Tyr807Ser, a peptide that harbors a mutation that disrupts receptor clustering, displayed markedly reduced interactions. Clustering motif peptides interacted with clathrin cages assembled in the presence or absence of AP2, with recombinant clathrin terminal domains, but not with clathrin hubs. The identification of terminal domains as the primary site of interaction for FXNPXY807 suggests that adaptor molecules are not required for receptor-mediated endocytosis of LDL, and that at least two different tyrosine-based internalization motifs exist for clustering receptors in coated pits.

Original languageEnglish (US)
Pages (from-to)59-67
Number of pages9
JournalJournal of Cell Biology
Volume142
Issue number1
DOIs
StatePublished - Jul 13 1998

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Clathrin
LDL Receptors
Cluster Analysis
Peptides
Endocytosis
Tyrosine
Magnetic Resonance Spectroscopy
Mutation

Keywords

  • Clathrin
  • Endocytosis
  • LDL receptor
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Cell Biology

Cite this

The LDL receptor clustering motif interacts with the clathrin terminal domain in a reverse turn conformation. / Kibbey, Richard G.; Rizo-Rey, Jose; Gierasch, Lila M.; Anderson, Richard G W.

In: Journal of Cell Biology, Vol. 142, No. 1, 13.07.1998, p. 59-67.

Research output: Contribution to journalArticle

Kibbey, Richard G. ; Rizo-Rey, Jose ; Gierasch, Lila M. ; Anderson, Richard G W. / The LDL receptor clustering motif interacts with the clathrin terminal domain in a reverse turn conformation. In: Journal of Cell Biology. 1998 ; Vol. 142, No. 1. pp. 59-67.
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