The LDL-receptor-related protein, LRP, is an apolipoprotein E-binding protein

Ulrike Beisiegel, Wilfried Weber, Gudrun Ihrke, Joachim Herz, Keith K. Stanley

Research output: Contribution to journalArticle

523 Citations (Scopus)

Abstract

The low-density-lipoprotein (LDL) receptor is a cell-surface pro-tein that plays an important part in the metabolism of cholesterol by mediating the uptake of LDL from plasma into cells1. Although LDL particles bind to the LDL receptor through their apolipoprotein B (apo B) and apolipoprotein E (apo E) moieties, other apo E-containing particles, like chylomicron remnants, are not dependent on the LDL receptor for uptake into cells. Chylomicrons formed in the intestinal mucosa during the absorption of the products of digestion, are processed by the peripheral circula-tion by lipoprotein lipase, which catalyses the breakdown of triglycerides in chylomicrons to free fatty acids and glycerol. The resulting chylomicron remnants, which are cholesterol-rich liproproteins, are subsequently taken up in the liver2-6. A second distinct protein that binds to apo E-containing lipoproteins, but not to LDL, has been proposed to be the receptor mediating the clearance of chylomicron remnants from the plasma. This protein has a relative molecular mass (Mr) of 56,000 (56K) 7. More recent studies have failed, however, to establish whether this protein is a cell-surface receptor8. Here we describe crosslinking experiments in which apo E liposomes were found to bind specifically to the cell surface of hepG2 cells and to human liver membranes. The size and immunological cross-reactivity of the protein to which the liposomes bound was indistinguishable from that of the recently cloned and sequenced LDL-receptor-related protein, LRP9. We therefore conclude that the LRP might function as an apo E receptor.

Original languageEnglish (US)
Pages (from-to)162-164
Number of pages3
JournalNature
Volume341
Issue number6238
StatePublished - 1989

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LDL-Receptor Related Proteins
LDL Receptors
Chylomicron Remnants
Apolipoproteins E
Carrier Proteins
LDL Lipoproteins
Chylomicrons
Liposomes
Proteins
Cholesterol
Low Density Lipoprotein Receptor-Related Protein-1
Lipoprotein Lipase
Hep G2 Cells
Apolipoproteins B
Intestinal Mucosa
Nonesterified Fatty Acids
Glycerol
Lipoproteins
Digestion
Triglycerides

ASJC Scopus subject areas

  • General

Cite this

Beisiegel, U., Weber, W., Ihrke, G., Herz, J., & Stanley, K. K. (1989). The LDL-receptor-related protein, LRP, is an apolipoprotein E-binding protein. Nature, 341(6238), 162-164.

The LDL-receptor-related protein, LRP, is an apolipoprotein E-binding protein. / Beisiegel, Ulrike; Weber, Wilfried; Ihrke, Gudrun; Herz, Joachim; Stanley, Keith K.

In: Nature, Vol. 341, No. 6238, 1989, p. 162-164.

Research output: Contribution to journalArticle

Beisiegel, U, Weber, W, Ihrke, G, Herz, J & Stanley, KK 1989, 'The LDL-receptor-related protein, LRP, is an apolipoprotein E-binding protein', Nature, vol. 341, no. 6238, pp. 162-164.
Beisiegel U, Weber W, Ihrke G, Herz J, Stanley KK. The LDL-receptor-related protein, LRP, is an apolipoprotein E-binding protein. Nature. 1989;341(6238):162-164.
Beisiegel, Ulrike ; Weber, Wilfried ; Ihrke, Gudrun ; Herz, Joachim ; Stanley, Keith K. / The LDL-receptor-related protein, LRP, is an apolipoprotein E-binding protein. In: Nature. 1989 ; Vol. 341, No. 6238. pp. 162-164.
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