The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coli

Benjamin J. Habdas, Jennifer Smart, James B. Kaper, Vanessa Sperandio

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 responds to the host-produced epinephrine and norepinephrine, and bacterially produced autoinducer 3 (AI-3), through two-component systems. Further integration of multiple regulatory signaling networks, involving regulators such as the LysR-type transcriptional regulator (LTTR) QseA, promotes effective regulation of virulence factors. These include the production of flagella, a phage-encoded Shiga toxin, and genes within the locus of enterocyte effacement (LEE) responsible for attaching and effacing (AE) lesion formation. Here, we describe a new member of this signaling cascade, an LTTR heretofore renamed QseD (quorum-sensing E. coli regulator D). QseD is present in all enterobacteria but exists almost exclusively in O157:H7 isolates as a helix-turn-helix (HTH) truncated isoform. This "short" isoform (sQseD) is still able to regulate gene expression through a different mechanism than the full-length K-12 E. coli "long" QseD isoform (lQseD). The EHEC ΔqseD mutant exhibits increased expression of all LEE operons and deregulation of AE lesion formation. The loss of qseD in EHEC does not affect motility, but the K-12 ΔqseD mutant is hypermotile. While the lQseD directly binds to the ler promoter, encoding the LEE master regulator, to repress LEE transcription, the sQseD isoform does not. LTTRs bind to DNA as tetramers, and these data suggest that sQseD regulates ler by forming heterotetramers with another LTTR. The LTTRs known to regulate LEE transcription, QseA and LrhA, do not interact with sQseD, suggesting that sQseD acts as a dominant-negative partner with a yet-unidentified LTTR.

Original languageEnglish (US)
Pages (from-to)3699-3712
Number of pages14
JournalJournal of Bacteriology
Volume192
Issue number14
DOIs
StatePublished - Jul 2010

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Enterohemorrhagic Escherichia coli
Quorum Sensing
Enterocytes
Protein Isoforms
Escherichia coli
Shiga Toxin
Escherichia coli O157
Flagella
Virulence Factors
Enterobacteriaceae
Operon
Bacteriophages
Epinephrine
Norepinephrine
Gene Expression
DNA
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coli. / Habdas, Benjamin J.; Smart, Jennifer; Kaper, James B.; Sperandio, Vanessa.

In: Journal of Bacteriology, Vol. 192, No. 14, 07.2010, p. 3699-3712.

Research output: Contribution to journalArticle

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