The mechanism of noradrenergic α1 excitatory modulation of pontine reticular formation neurons

D. R. Stevens, R. W. McCarley, R. W. Greene

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The α1 adrenergic receptor occurs in all major divisions of the CNS and is thought to play a role in all behaviors influenced by norepinephrine (NE). In the medial pontine reticular formation (mPRF), the proposed site of adrenergic enhancement of startle responses (Davis, 1984), α1 agonists excite most neurons (Gerber et al., 1990). We here report that α1 excitation results from a reduction of a voltage- and calcium-dependent potassium current, not previously recognized as ligand-modulated. The calcium sensitivity is suggested by its antagonism with Mg2+, Cd2+, Ba2+, low concentrations of tetraethylammonium, and charybdotoxin. The voltage sensitivity of this conductance falls within the membrane potential range critical to action potential generation. Based on this voltage sensitivity, the change in repetitive firing characteristics may be predicted according to a mathematical model of the mPRF neuronal electrophysiology. The predicted response to a 50% decrease in the phenylephrine (PE)-sensitive conductance is similar to the observed responses, with respect to both the current response under voltage-clamp conditions and alterations of the AHP and frequency/current curve. In contrast, modeling a reduction of a voltage- insensitive leak current predicts none of these changes. Thus, the noradrenergic reduction of this current depolarizes the membrane, increases the likelihood of an initial response to depolarizing input, and increases firing rate during sustained depolarization in a manner consistent with an NE role as an excitatory neuromodulator of the mPRF.

Original languageEnglish (US)
Pages (from-to)6481-6487
Number of pages7
JournalJournal of Neuroscience
Volume14
Issue number11 I
StatePublished - 1994

Fingerprint

Neurons
Norepinephrine
Startle Reflex
Charybdotoxin
Calcium
Tetraethylammonium
Electrophysiology
Phenylephrine
Adrenergic Agents
Membrane Potentials
Adrenergic Receptors
Action Potentials
Neurotransmitter Agents
Potassium
Theoretical Models
Ligands
Membranes
Pontine Tegmentum

Keywords

  • calcium dependence
  • neuronal simulation
  • phenylephrine
  • potassium channels
  • startle response

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The mechanism of noradrenergic α1 excitatory modulation of pontine reticular formation neurons. / Stevens, D. R.; McCarley, R. W.; Greene, R. W.

In: Journal of Neuroscience, Vol. 14, No. 11 I, 1994, p. 6481-6487.

Research output: Contribution to journalArticle

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