The Mechanisms of PML-Nuclear Body Formation

Tian Huai Shen, Hui Kuan Lin, Pier Paolo Scaglioni, Thomas M. Yung, Pier Paolo Pandolfi

Research output: Contribution to journalArticle

360 Scopus citations

Abstract

PML nuclear bodies (NBs) are nuclear structures that have been implicated in processes such as transcriptional regulation, genome stability, response to viral infection, apoptosis, and tumor suppression. PML has been found to be essential for the formation of the NBs, as these structures do not form in Pml null cells, although PML add back fully rescues their formation. However, the basis for such a structural role of PML is unknown. We demonstrate that PML contains a SUMO binding motif that is independent of its SUMOylation sites and is surprisingly necessary for PML-NB formation. We demonstrate that the PML RING domain is critical for PML SUMOylation and PML-NB formation. We propose a model for PML-NB formation whereby PML SUMOylation and noncovalent binding of PML to SUMOylated PML through the SUMO binding motif constitutes the nucleation event for subsequent recruitment of SUMOylated proteins and/or proteins containing SUMO binding motifs to the PML NBs.

Original languageEnglish (US)
Pages (from-to)331-339
Number of pages9
JournalMolecular cell
Volume24
Issue number3
DOIs
StatePublished - Nov 3 2006

Keywords

  • CELLBIO
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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