@article{f61b0031ebcd4adb980df5e67f58f40c,
title = "The mechanosensitive ion channel traak is localized to the mammalian node of ranvier",
abstract = "TRAAK is a membrane tension-activated K+ channel that has been associated through behavioral studies to mechanical nociception. We used specific monoclonal antibodies in mice to show that TRAAK is localized exclusively to nodes of Ranvier, the action potential propagating elements of myelinated nerve fibers. Approximately 80 percent of myelinated nerve fibers throughout the central and peripheral nervous system contain TRAAK in what is likely an all-nodes or no-nodes per axon fashion. TRAAK is not observed at the axon initial segment where action potentials are first generated. We used polyclonal antibodies, the TRAAK inhibitor RU2 and node clamp amplifiers to demonstrate the presence and functional properties of TRAAK in rat nerve fibers. TRAAK contributes to the {\textquoteleft}leak{\textquoteright} K+ current in mammalian nerve fiber conduction by hyperpolarizing the resting membrane potential, thereby increasing Na+ channel availability for action potential propagation. We speculate on why nodes of Ranvier contain a mechanosensitive K+ channel.",
author = "Brohawn, {Stephen G.} and Weiwei Wang and Annie Handler and Campbell, {Ernest B.} and Schwarz, {J{\"u}rgen R.} and Roderick MacKinnon",
note = "Funding Information: JRS wants to thank the Deutsche Forschungsgemeinschaft for financial support (Schw 292/17?1), Yvonne Pechmann for unfailing technical assistance, and Dr. Matthias Kneussel for continuous sup- port. We thank staff at APS beamline 24-IDC/E, especially K Rajashankar, for assistance at the synchrotron, staff at the Rockefeller Bio-Imaging Resource Center for imaging support, N Renier, Z Wu, D Simon, and M Tessier-Lavigne for advice on iDISCO experiments, M Lazdunski and F Lesage for the TRAAK/TREK KO mice from which the TRAAK KO mouse line was generated, and Dr. F Sigworth for helpful discussions on designing of the opamp based node-clamp and N Hu for a discussion on conditional probabilities. RM is an Investigator in the Howard Hughes Medical Institute. Funding Information: JRS wants to thank the Deutsche Forschungsgemeinschaft for financial support (Schw 292/17–1), Yvonne Pechmann for unfailing technical assistance, and Dr. Matthias Kneussel for continuous support. We thank staff at APS beamline 24-IDC/E, especially K Rajashankar, for assistance at the synchrotron, staff at the Rockefeller Bio-Imaging Resource Center for imaging support, N Renier, Z Wu, D Simon, and M Tessier-Lavigne for advice on iDISCO experiments, M Lazdunski and F Lesage for the TRAAK/TREK KO mice from which the TRAAK KO mouse line was generated, and Dr. F Sigworth for helpful discussions on designing of the opamp based node-clamp and N Hu for a discussion on conditional probabilities. RM is an Investigator in the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2019, eLife Sciences Publications Ltd. All rights reserved.",
year = "2019",
month = nov,
doi = "10.7554/eLife.50403",
language = "English (US)",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}