The membrane-bound electron-transfer components of aerobically grown Chromatium vinosum

R. Max Wynn; Charlotte Kämpf; Dale F. Gaul; Won Ki Choi; Robert W. Shaw; David B. Knaff

doi:10.1016/0005-2728(85)90030-1

The membrane-bound electron-transfer components of aerobically grown Chromatium vinosum

R. Max Wynn, Charlotte Kämpf, Dale F. Gaul, Won Ki Choi, Robert W. Shaw, David B. Knaff

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The electron-transfer chain components of aerobically grown Chromatium vinosum have been characterized. Membranes isolated from aerobically grown C. vinosum have been shown to contain a Rieske iron-sulfur protein, at least three c-type cytochromes and at least one b-type cytochrome. Two cytochromes that bind CO appear to be present, one of which may function as a terminal oxidase. Membranes isolated from these cells appear to lack a photochemical reaction center and the high potential (E_m = +340 _mV) cytochrome c-555 that serves as the immediate donor to the reaction center in photosynthetically grown C. vinosum. In addition, the b-cytochrome(s) of aerobically grown C. vinosum has (have) been shown to be considerably more electronegative (E′_m = -90 _mV) than that of photosynthetically grown cells (E′_m = +30 _mV).

Original language	English (US)
Pages (from-to)	85-93
Number of pages	9
Journal	BBA - Bioenergetics
Volume	808
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(85)90030-1
State	Published - Jun 26 1985

Keywords

(C. vinosum)
Cytochrome
Electron transport
Iron-sulfur protein

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(85)90030-1

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title = "The membrane-bound electron-transfer components of aerobically grown Chromatium vinosum",

abstract = "The electron-transfer chain components of aerobically grown Chromatium vinosum have been characterized. Membranes isolated from aerobically grown C. vinosum have been shown to contain a Rieske iron-sulfur protein, at least three c-type cytochromes and at least one b-type cytochrome. Two cytochromes that bind CO appear to be present, one of which may function as a terminal oxidase. Membranes isolated from these cells appear to lack a photochemical reaction center and the high potential (Em = +340 mV) cytochrome c-555 that serves as the immediate donor to the reaction center in photosynthetically grown C. vinosum. In addition, the b-cytochrome(s) of aerobically grown C. vinosum has (have) been shown to be considerably more electronegative (E′m = -90 mV) than that of photosynthetically grown cells (E′m = +30 mV).",

keywords = "(C. vinosum), Cytochrome, Electron transport, Iron-sulfur protein",

author = "Wynn, {R. Max} and Charlotte K{\"a}mpf and Gaul, {Dale F.} and Choi, {Won Ki} and Shaw, {Robert W.} and Knaff, {David B.}",

note = "Funding Information: The authors wish to thank Professor Norbert Pfennig for his assistance in supplying cells of aerobically grown C. vinosum, and communicating unpublished results. This work was supported, in part, by grants (to D.B.K.) from the National Science Foundation (PCM-8109635 and PCM-8408564) and the Texas Tech Institute for University Research and (to R.W.S.) from the Robert A. Welch Foundation (D-909).",

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AU - Wynn, R. Max

AU - Kämpf, Charlotte

AU - Gaul, Dale F.

AU - Choi, Won Ki

AU - Shaw, Robert W.

AU - Knaff, David B.

N1 - Funding Information: The authors wish to thank Professor Norbert Pfennig for his assistance in supplying cells of aerobically grown C. vinosum, and communicating unpublished results. This work was supported, in part, by grants (to D.B.K.) from the National Science Foundation (PCM-8109635 and PCM-8408564) and the Texas Tech Institute for University Research and (to R.W.S.) from the Robert A. Welch Foundation (D-909).

PY - 1985/6/26

Y1 - 1985/6/26

N2 - The electron-transfer chain components of aerobically grown Chromatium vinosum have been characterized. Membranes isolated from aerobically grown C. vinosum have been shown to contain a Rieske iron-sulfur protein, at least three c-type cytochromes and at least one b-type cytochrome. Two cytochromes that bind CO appear to be present, one of which may function as a terminal oxidase. Membranes isolated from these cells appear to lack a photochemical reaction center and the high potential (Em = +340 mV) cytochrome c-555 that serves as the immediate donor to the reaction center in photosynthetically grown C. vinosum. In addition, the b-cytochrome(s) of aerobically grown C. vinosum has (have) been shown to be considerably more electronegative (E′m = -90 mV) than that of photosynthetically grown cells (E′m = +30 mV).

AB - The electron-transfer chain components of aerobically grown Chromatium vinosum have been characterized. Membranes isolated from aerobically grown C. vinosum have been shown to contain a Rieske iron-sulfur protein, at least three c-type cytochromes and at least one b-type cytochrome. Two cytochromes that bind CO appear to be present, one of which may function as a terminal oxidase. Membranes isolated from these cells appear to lack a photochemical reaction center and the high potential (Em = +340 mV) cytochrome c-555 that serves as the immediate donor to the reaction center in photosynthetically grown C. vinosum. In addition, the b-cytochrome(s) of aerobically grown C. vinosum has (have) been shown to be considerably more electronegative (E′m = -90 mV) than that of photosynthetically grown cells (E′m = +30 mV).

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The membrane-bound electron-transfer components of aerobically grown Chromatium vinosum

Abstract

Keywords

ASJC Scopus subject areas

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