The membrane-bound transcription factor CREB3L1 is activated in response to virus infection to inhibit proliferation of virus-infected cells

Bray Denard, Joachim Seemann, Qiuyue Chen, Austin Gay, Hua Huang, Yan Chen, Jin Ye

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

CREB3L1/OASIS is a cellular transcription factor synthesized as a membrane-bound precursor and activated by regulated intramembrane proteolysis in response to stimuli like ER stress. Comparing gene expression between Huh7 subclones that are permissive for hepatitis C virus (HCV) replication versus the nonpermissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, including murine γ-herpesvirus 68, HCV, West Nile virus (WNV), and Sendai virus, CREB3L1 was proteolytically cleaved, allowing its NH 2 terminus to enter the nucleus and induce multiple genes encoding inhibitors of the cell cycle to block cell proliferation. Consistent with this, we observed a necessity for CREB3L1 expression to be silenced in proliferating cells that harbor replicons of HCV or WNV. Our results indicate that CREB3L1 may play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells.

Original languageEnglish (US)
Pages (from-to)65-74
Number of pages10
JournalCell Host and Microbe
Volume10
Issue number1
DOIs
StatePublished - Jul 21 2011

Fingerprint

Virus Diseases
Transcription Factors
Hepacivirus
Viruses
West Nile virus
Membranes
Rhadinovirus
Sendai virus
Replicon
DNA Viruses
RNA Viruses
Virus Replication
Proteolysis
Cell Cycle
Cell Proliferation
Gene Expression
Infection
Genes

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

The membrane-bound transcription factor CREB3L1 is activated in response to virus infection to inhibit proliferation of virus-infected cells. / Denard, Bray; Seemann, Joachim; Chen, Qiuyue; Gay, Austin; Huang, Hua; Chen, Yan; Ye, Jin.

In: Cell Host and Microbe, Vol. 10, No. 1, 21.07.2011, p. 65-74.

Research output: Contribution to journalArticle

@article{205e8ef15a534445ab7511b3dbb1c4aa,
title = "The membrane-bound transcription factor CREB3L1 is activated in response to virus infection to inhibit proliferation of virus-infected cells",
abstract = "CREB3L1/OASIS is a cellular transcription factor synthesized as a membrane-bound precursor and activated by regulated intramembrane proteolysis in response to stimuli like ER stress. Comparing gene expression between Huh7 subclones that are permissive for hepatitis C virus (HCV) replication versus the nonpermissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, including murine γ-herpesvirus 68, HCV, West Nile virus (WNV), and Sendai virus, CREB3L1 was proteolytically cleaved, allowing its NH 2 terminus to enter the nucleus and induce multiple genes encoding inhibitors of the cell cycle to block cell proliferation. Consistent with this, we observed a necessity for CREB3L1 expression to be silenced in proliferating cells that harbor replicons of HCV or WNV. Our results indicate that CREB3L1 may play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells.",
author = "Bray Denard and Joachim Seemann and Qiuyue Chen and Austin Gay and Hua Huang and Yan Chen and Jin Ye",
year = "2011",
month = "7",
day = "21",
doi = "10.1016/j.chom.2011.06.006",
language = "English (US)",
volume = "10",
pages = "65--74",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - The membrane-bound transcription factor CREB3L1 is activated in response to virus infection to inhibit proliferation of virus-infected cells

AU - Denard, Bray

AU - Seemann, Joachim

AU - Chen, Qiuyue

AU - Gay, Austin

AU - Huang, Hua

AU - Chen, Yan

AU - Ye, Jin

PY - 2011/7/21

Y1 - 2011/7/21

N2 - CREB3L1/OASIS is a cellular transcription factor synthesized as a membrane-bound precursor and activated by regulated intramembrane proteolysis in response to stimuli like ER stress. Comparing gene expression between Huh7 subclones that are permissive for hepatitis C virus (HCV) replication versus the nonpermissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, including murine γ-herpesvirus 68, HCV, West Nile virus (WNV), and Sendai virus, CREB3L1 was proteolytically cleaved, allowing its NH 2 terminus to enter the nucleus and induce multiple genes encoding inhibitors of the cell cycle to block cell proliferation. Consistent with this, we observed a necessity for CREB3L1 expression to be silenced in proliferating cells that harbor replicons of HCV or WNV. Our results indicate that CREB3L1 may play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells.

AB - CREB3L1/OASIS is a cellular transcription factor synthesized as a membrane-bound precursor and activated by regulated intramembrane proteolysis in response to stimuli like ER stress. Comparing gene expression between Huh7 subclones that are permissive for hepatitis C virus (HCV) replication versus the nonpermissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, including murine γ-herpesvirus 68, HCV, West Nile virus (WNV), and Sendai virus, CREB3L1 was proteolytically cleaved, allowing its NH 2 terminus to enter the nucleus and induce multiple genes encoding inhibitors of the cell cycle to block cell proliferation. Consistent with this, we observed a necessity for CREB3L1 expression to be silenced in proliferating cells that harbor replicons of HCV or WNV. Our results indicate that CREB3L1 may play an important role in limiting virus spread by inhibiting proliferation of virus-infected cells.

UR - http://www.scopus.com/inward/record.url?scp=79960546549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960546549&partnerID=8YFLogxK

U2 - 10.1016/j.chom.2011.06.006

DO - 10.1016/j.chom.2011.06.006

M3 - Article

C2 - 21767813

AN - SCOPUS:79960546549

VL - 10

SP - 65

EP - 74

JO - Cell Host and Microbe

JF - Cell Host and Microbe

SN - 1931-3128

IS - 1

ER -