The method of quantitative burn wound biopsy cultures and its routine use in the care of the burned patient

E. C. Loebl, J. A. Marvin, E. L. Heck, P. W. Curreri, C. R. Baxter

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

A method for quantitative biopsy cultures of burn wounds has been developed and evaluated. Specimens are obtained by full thickness biopsy of the burn wound using a scalpel. Specimens are processed by maceration, suspension in physiologic saline solution, and plating of serial dilutions of the suspension on appropriate media. Quantitative counts are performed and expressed as the number of bacteria per 9. of burn wound tissue. Experience with this technic in the care of 210 consecutive patients with burns of more than 20% total body surface area has shown it to be more reliable than surface culture technics. Patients were considered to have wound colonization when 10 4 or more organisms per Gm. of burned tissue were recovered from different areas of the body. Appropriate antibiotic therapy was instituted on the basis of these results. Routine use of this method was found to be effective, simple, and practical as an aid in the early diagnosis of sepsis in the burned patient.

Original languageEnglish (US)
Pages (from-to)20-24
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume61
Issue number1
StatePublished - 1974

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Patient Care
Biopsy
Wounds and Injuries
Suspensions
Body Surface Area
Burns
Sodium Chloride
Early Diagnosis
Sepsis
Anti-Bacterial Agents
Bacteria
Therapeutics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Loebl, E. C., Marvin, J. A., Heck, E. L., Curreri, P. W., & Baxter, C. R. (1974). The method of quantitative burn wound biopsy cultures and its routine use in the care of the burned patient. American Journal of Clinical Pathology, 61(1), 20-24.

The method of quantitative burn wound biopsy cultures and its routine use in the care of the burned patient. / Loebl, E. C.; Marvin, J. A.; Heck, E. L.; Curreri, P. W.; Baxter, C. R.

In: American Journal of Clinical Pathology, Vol. 61, No. 1, 1974, p. 20-24.

Research output: Contribution to journalArticle

Loebl, E. C. ; Marvin, J. A. ; Heck, E. L. ; Curreri, P. W. ; Baxter, C. R. / The method of quantitative burn wound biopsy cultures and its routine use in the care of the burned patient. In: American Journal of Clinical Pathology. 1974 ; Vol. 61, No. 1. pp. 20-24.
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