The mitotic spindle mediates inheritance of the Golgi ribbon structure

Jen Hsuan Wei, Joachim Seemann

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The mammalian Golgi ribbon disassembles during mitosis and reforms in both daughter cells after division. Mitotic Golgi membranes concentrate around the spindle poles, suggesting that the spindle may control Golgi partitioning. To test this, cells were induced to divide asymmetrically with the entire spindle segregated into only one daughter cell. A ribbon reforms in the nucleated karyoplasts, whereas the Golgi stacks in the cytoplasts are scattered. However, the scattered Golgi stacks are polarized and transport cargo. Microinjection of Golgi extract together with tubulin or incorporation of spindle materials rescues Golgi ribbon formation. Therefore, the factors required for postmitotic Golgi ribbon assembly are transferred by the spindle, but the constituents of functional stacks are partitioned independently, suggesting that Golgi inheritance is regulated by two distinct mechanisms.

Original languageEnglish (US)
Pages (from-to)391-397
Number of pages7
JournalJournal of Cell Biology
Volume184
Issue number3
DOIs
StatePublished - Feb 9 2009

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Spindle Apparatus
Spindle Poles
Microinjections
Tubulin
Mitosis
Cell Division
Membranes

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

The mitotic spindle mediates inheritance of the Golgi ribbon structure. / Wei, Jen Hsuan; Seemann, Joachim.

In: Journal of Cell Biology, Vol. 184, No. 3, 09.02.2009, p. 391-397.

Research output: Contribution to journalArticle

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